What is the µ-Plate 24 Well Glass Bottom?

A 24 well plate with black walls and a flat No. 1.5H glass coverslip bottom, 170 µm ± 5 µm, in Schott D 263 M borosilicate. Each well is 14.0 mm across with 1.54 cm² of growth area and 1 ml of medium. The footprint follows ANSI/SLAS 1 to 4:2004, so it goes into a plate reader or a screening platform without an adapter.

Flatness and black walls

Two things separate an imaging plate from an ordinary culture plate. Inner well flatness and whole plate flatness decide whether an automated microscope holds focus across all 24 wells, or refocuses in every one. Black walls cut the light bleeding from one well into the next during fluorescence reads. Both matter more as the run gets longer and more automated.

Where I would not spend the money

The polymer bottom version, 82426, is the same plate with a #1.5 polymer coverslip and costs less. For widefield fluorescence and confocal it is the sensible buy. The glass version earns its price for TIRF, single molecule detection and super-resolution. One more thing worth knowing before you order: the glass bottom is fragile and breaks easily, so if the plate is going through a liquid handler with a hard deck, the polymer version is the safer choice.

Key Features

• No. 1.5H glass coverslip bottom, 170 µm ± 5 µm, Schott D 263 M
• 24 wells, 14.0 mm inner diameter, 1.54 cm² and 1 ml each
• Black walls for low well to well crosstalk
• ANSI/SLAS 1 to 4:2004 footprint, inner well and whole plate flatness for automated screening
• Sterilized, 15 or 90 per box