ibidi µ-Dish 35 mm Quad

One 35 mm dish divided into four moulded compartments, 0.85 cm² and 300 µl each, on the same #1.5 polymer coverslip bottom as the rest of the range. Four conditions in the incubator footprint of one dish, and the Ph+ compartment geometry keeps phase contrast usable right out to the wall.

ibidi µ-Dish 35 mm Quad

What is the µ-Dish 35 mm Quad?

A 35 mm dish split into four moulded compartments, each with 0.85 cm² of growth area and 300 µl of medium, on the same #1.5 ibidi Polymer Coverslip bottom as the rest of the range. Four experiments in the space and the incubator footprint of one dish.

The compartments are moulded, not a removable insert, so there is no gap assay here and nothing to lift out. This is for running four conditions side by side and imaging them in one session.

The Ph+ walls

Ph+ is ibidi's feature for suppressing the meniscus, and the meniscus is what wrecks phase contrast in an ordinary open well. On a compartment holding 300 µl the meniscus is a large fraction of the surface, so this matters more here than it would in a 6 well plate. Anyone who has tried to score cells at the edge of a small well knows what the alternative looks like.

Where I would not spend the money

Four compartments in one dish is convenient, and it is not four dishes. They share a lid and a base, so a contamination in one compartment is a problem for all four, and you cannot pull one out mid experiment without opening the others. For four genuinely independent conditions, four separate µ-Dishes are safer, and the price difference is small. Buy the Quad when the four conditions belong together and you want them under one objective in one run.

Key Features

  • Four moulded compartments, 0.85 cm² and 300 µl each
  • Ph+ meniscus suppression for phase contrast across the whole compartment
  • #1.5 ibidi Polymer Coverslip bottom, 180 µm, refractive index 1.52
  • ibiTreat (80416) or uncoated (80411)
  • Sterilized, 60 or 400 per box, 36 month shelf life

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Catalog number80416 ibiTreat, 80411 uncoated
Compartments4
Growth area per compartment0.85 cm²
Coating area per compartment2.46 cm²
Volume per compartment300 µl
Dish diameter35 mm
Height with lid12 mm
Height without lid10 mm
Coverslip#1.5 ibidi Polymer, 180 µm (+10/-5 µm)
Refractive index at 589 nm1.52
Abbe number56
Optical propertiesExtremely low birefringence and autofluorescence
SurfaceibiTreat tissue culture treated, or uncoated
SterilizationSterilized, sealed in gas permeable packaging
TemperatureStable to 80 °C, not autoclavable
StorageRT (15 to 25 °C), dry, no direct sunlight
Shelf life36 months
Pieces per box60 or 400, individually packed
ManufacturedGermany

Applications

  • Four parallel cellular assays in one dish
  • Live cell imaging
  • Phase contrast microscopy
  • Fluorescence and confocal imaging
  • Immunofluorescence staining

ibidi u-Dish 35 mm Quad seen from above showing the four compartments

Cell Cultivation and Imaging

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Fluorescence Imaging

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Research publications using ibidi microfluidic devices and culture formats

Comprehensive collection of peer-reviewed research publications demonstrating the use of ibidi labware including µ-Slides, µ-Dishes, µ-Plates, and specialized microfluidic devices for cell biology, neurobiology, vascular biology, and cancer research.

Are the compartments removable?

No. They are moulded into the dish. If you want a removable divider that leaves a defined cell free gap, that is the Culture-Insert range, not this.

What is Ph+?

ibidi's name for the compartment geometry that keeps phase contrast usable across the whole compartment and cell growth even within it. On a compartment holding 300 µl, the edge is where phase contrast normally falls apart.

Quad, or four separate dishes?

The four compartments share a lid and a base. A contamination in one is a problem for all four, and you cannot remove one mid experiment without opening the others. For four genuinely independent conditions, four separate µ-Dishes are the safer buy and the price difference is small. The Quad earns its place when the four conditions belong together and you want them under one objective in one run.

80416 or 80411?

80416 is ibiTreat, tissue culture treated. 80411 is uncoated. Same dish otherwise.

Is it available in Israel with local support?

Yes. NBT is the ibidi distributor in Israel. We hold labware locally, and we will tell you when a cheaper item in the range does the same job.

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