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ibidi µ-Slide 4 Well
All-in-one chamber slide with 4 wells used for cell culture and high-end microscopy.
A cell culture chamber for observation of the sample through the polymer coverslip bottom using high resolution microscopy.A microscopy slide for fast and easy, no coverslips and no leakage for cost-effective experiments using small numbers of cells and low volumes of reagents.
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Technical Features
- Open µ-Slide (chambered coverslip) with 4 independent wells
- Excellent optical quality imaging chamber for high-end microscopy
- Compatible with staining and fixation
- ibiTreat surface for optimal cell adhesion
- Made of biocompatible plastic material – no glue and no leaking
- Minimal mirror effect on the µ-Slide walls for light sheet microscopy
- Also available with a glass bottom
- Available with a non-adhesive Bioinert surface: µ-Slide 4 Well Bioinert, specifically suitable for 3D applications, such as spheroids and suspension cells
Experimental Example
Immunofluorescence of Human Umbilical Vein Endothelial Cells (HUVEC) in a µ-Slide 4 Well
Blue: Nucleus (DAPI)
Green: VE-Cadherin (Alexa555)
Red: F-Actin (Alexa488)
Meniscus-Free Phase Contrast Microscopy
µ-Slide 4 Well
Top View
The µ-Slide 4 Well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 4 Well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.
This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of well contrasted cells
The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam
Cell Cultivation and Imaging
Explore All ProductsFluorescence Imaging
Explore All Productsibidi Labware Publications
Comprehensive collection of peer-reviewed research publications demonstrating the use of ibidi labware including µ-Slides, µ-Dishes, µ-Plates, and specialized microfluidic devices for cell biology, neurobiology, vascular biology, and cancer research.
What is the µ-Slide 4 Well used for?
Four-chamber slide with 700 µL per well for parallel cell culture experiments and immunofluorescence assays. Independent wells with #1.5 Polymer or Glass Coverslip bottom enable simultaneous multi-condition microscopy in a standard slide footprint.
Is it compatible with high-resolution microscopy?
Yes — features ibidi's premium #1.5 polymer coverslip bottom, ideal for widefield fluorescence, DIC, phase contrast, and confocal imaging. For super-resolution and TIRF, choose the Glass Bottom variant.
Is the µ-Slide 4 Well sterile and ready to use?
Yes. All ibidi labware ships sterilized and individually wrapped, ready for direct cell seeding without additional preparation.
What surface treatment options are available for the µ-Slide 4 Well?
ibidi labware is offered in multiple surface treatments including ibiTreat (tissue-culture treated), Bioinert, hydrophobic, and uncoated — selected to match adherent or suspension cell requirements.
Is the µ-Slide 4 Well available with local Israeli support?
Yes. NBT Ltd is the exclusive ibidi distributor in Israel, providing application training, installation guidance, and full local technical support for the µ-Slide 4 Well.
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