ibidi µ-Slide 4 Well

All-in-one chamber slide with 4 wells used for cell culture and high-end microscopy.

ibidi µ-Slide 4 Well

A cell culture chamber for observation of the sample through the polymer coverslip bottom using high resolution microscopy.A microscopy slide for fast and easy, no coverslips and no leakage for cost-effective experiments using small numbers of cells and low volumes of reagents.

Technical Features

  • Open µ-Slide (chambered coverslip) with 4 independent wells
  • Excellent optical quality imaging chamber for high-end microscopy
  • Compatible with staining and fixation
  • ibiTreat surface for optimal cell adhesion
  • Made of biocompatible plastic material – no glue and no leaking
  • Minimal mirror effect on the µ-Slide walls for light sheet microscopy
  • Also available with a glass bottom
  • Available with a non-adhesive Bioinert surface: µ-Slide 4 Well Bioinert, specifically suitable for 3D applications, such as spheroids and suspension cells

Experimental Example

Immunofluorescence of Human Umbilical Vein Endothelial Cells (HUVEC) in a µ-Slide 4 Well

Blue: Nucleus (DAPI)
Green: VE-Cadherin (Alexa555)
Red: F-Actin (Alexa488)

Meniscus-Free Phase Contrast Microscopy

µ-Slide 4 Well

µ-Slide 4 Well Ph+

Top View

The µ-Slide 4 Well Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 4 Well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of well contrasted cells

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 Well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam

Specifications

Outer dimensions (w x l) 25.5 x 75.5 mm²
Number of wells 4
Dimensions of wells
(w x l x h)
21.6 x 11.4 x 9.3 mm³
Volume per well 700 µl
Total height with / without lid 10.8 / 9.5 mm
Growth area per well 2.5 cm²
Coating area per well 4.1 cm²
Bottom: ibidi Polymer Coverslip

Cell Cultivation and Imaging

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Fluorescence Imaging

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ibidi

Academic publications and research studies utilizing ibidi microfluidic cell culture systems, sourced from Google Scholar.

What is the ibidi µ-Slide 4 Well and what is it used for?

The ibidi µ-Slide 4 Well is All-in-one chamber slide with 4 wells used for cell culture and high-end microscopy.

What research applications is the ibidi µ-Slide 4 Well suited for?

The ibidi µ-Slide 4 Well is suited for neuroscience, behavioral science, physiology, and related life science research workflows requiring reliable, high-quality data acquisition and analysis.

What are the key technical features of the ibidi µ-Slide 4 Well?

The ibidi µ-Slide 4 Well (catalog number: IBD-80426) is engineered for precision performance in laboratory settings, offering user-friendly operation, robust construction, and compatibility with standard research workflows.

Is the ibidi µ-Slide 4 Well available in Israel with local support?

Yes. NBT Ltd is the exclusive distributor of this product in Israel. Our team provides full local support including installation, application training, and technical assistance for the ibidi µ-Slide 4 Well.

What accessories or systems is the ibidi µ-Slide 4 Well compatible with?

The ibidi µ-Slide 4 Well is designed to integrate with complementary instruments and software in its application domain. NBT Ltd can advise on compatible accessories and system configurations for your research needs.

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