A cell culture chamber for observation of the sample through the polymer coverslip bottom using high resolution microscopy.A microscopy slide for fast and easy, no coverslips and no leakage for cost-effective experiments using small numbers of cells and low volumes of reagents.

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Technical Features

• Open µ-Slide (chambered coverslip) with 4 independent wells
• Excellent optical quality imaging chamber for high-end microscopy
• Compatible with staining and fixation
• ibiTreat surface for optimal cell adhesion
• Made of biocompatible plastic material – no glue and no leaking
• Minimal mirror effect on the µ-Slide walls for light sheet microscopy
• Also available with a glass bottom
• Available with a non-adhesive Bioinert surface: µ-Slide 4 Well Bioinert, specifically suitable for 3D applications, such as spheroids and suspension cells

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Experimental Example

Immunofluorescence of Human Umbilical Vein Endothelial Cells (HUVEC) in a µ-Slide 4 Well

Blue: Nucleus (DAPI)
Green: VE-Cadherin (Alexa555)
Red: F-Actin (Alexa488)

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Meniscus-Free Phase Contrast Microscopy

µ-Slide 4 Well

µ-Slide 4 Well ^Ph+

Top View

The µ-Slide 4 Well ^Ph+ (Phase Contrast +) is designed for excellent optical quality, especially for cell culture when normal phase contrast microscopy is being used. Opposed to the classic µ-Slide 4 Well, the Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus that disturbs the phase contrast effect in normal open wells. Openings near the corners provide access to the wells for easy filling and aspiration of liquids.

This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.

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Working with the µ-Slide 4 Well ^Ph+ diminishes the meniscus and increases the area of well contrasted cells

The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.

Working with the µ-Slide 4 Well ^Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam

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