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ibidi Gas Incubation System – Silver Line
A gas mixer with active humidity control for long-term live cell imaging, providing precise CO2 and O2 regulation with patented feedback-controlled humidity control to prevent evaporation. Pairs with any ibidi Heating System to form a complete Stage Top Incubator. Catalog Number: IBD-11923
What is the ibidi Gas Incubation System – Silver Line?
The ibidi Gas Incubation System – Silver Line is a precision gas mixer and active humidity control unit designed for long-term live cell imaging on the microscope. It provides independent control of CO2, O2, and relative humidity — maintaining stable physiological conditions throughout imaging sessions when paired with an ibidi Heating System.
The system includes the ibidi Gas Mixer – Silver Line, a Humidifying Column, and an optional Air Pressure Generator. Together with the ibidi Heating System, it forms the complete ibidi Stage Top Incubator for full environmental control on any inverted microscope stage.
Key Features
- Patented Humidity Control: Feedback-controlled humidity regulation maintains a constant, very high relative humidity level inside the Stage Top Incubator, completely preventing evaporation throughout long-term live cell imaging assays.
- CO2 and O2 Regulation: Independent control of CO2 (0–15%) and O2 (1–21%) with 0.1% resolution enables normoxia, hypoxia, and custom atmospheric conditions for demanding cell biology experiments.
- Compatible with All ibidi Heating Systems: Fully compatible with the ibidi Heating System Slide/Dish, ibidi Heating System Multiwell Plate, and ibidi Heating System 4 Slides — all Silver Line versions.
- Compact and Lab-Ready: The ibidi Gas Mixer (90 × 170 × 230 mm, 2230 g) fits easily on the lab bench and connects to standard pressurized air and CO2/N2 supply at 1 bar.
- Touch Display Compatible: Integrates with the optional ibidi Touch Display for real-time monitoring and data logging of CO2, O2, and humidity during incubation.
- Scalable Platform: Use as a standalone gas regulation unit or as part of the complete ibidi Stage Top Incubator for full atmospheric control on the microscope stage.
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Technical Specifications
ibidi Gas Mixer – Silver Line
| CO2 Control Range | 0–15% |
| CO2 Resolution | 0.1% |
| O2 Control Range | 1–21% |
| O2 Resolution | 0.1% |
| Flow Control (Mixed Gas) | 0–20 l/h |
| Humidity Control Range | 20–99% relative humidity |
| Humidity Resolution | 0.1% |
| Operating Temperature | 18–30°C / 64–86°F |
| Max. Operating Humidity | 80% relative humidity |
| Dimensions (w × l × h) | 90 × 170 × 230 mm |
| Weight | 2230 g / 4.9 lbs |
| Power Supply | AC 100–240 V, 50/60 Hz, 1.4 A |
| Gas Supply – Air | 1 bar optimum (0.4–1.2 bar / 5.8–17.4 psi) |
| Gas Supply – CO2 | 1 bar optimum (0.8–1.2 bar / 11.6–17.4 psi) |
Humidifying Column
| Max. Operating Humidity | 80% relative humidity |
| Dimensions (w × l × h) | 70 × 110 × 300 mm |
Applications
- Long-term live cell imaging under controlled CO2 and O2 atmospheres
- Hypoxia and normoxia experiments on the microscope stage
- Fluorescence, confocal, widefield, and TIRF microscopy
- Cell culture maintenance during live imaging assays
- Combined use with ibidi Heating Systems for complete stage top incubation
Cell Cultivation and Imaging
Explore All ProductsFluorescence Imaging
Explore All Productsibidi Gas Incubation System Publications
The ibidi Gas Incubation System is cited in studies requiring precise atmospheric control during live cell microscopy. Researchers use it for hypoxia assays, CO2/O2 regulation experiments, and long-term time-lapse imaging under physiological conditions.
What is the ibidi Gas Incubation System – Silver Line used for?
The Silver Line Gas Incubation System provides precise, programmable control of CO2 and O2 (including hypoxia and hyperoxia) inside an ibidi Stage Top Incubator during live-cell microscopy, enabling long-term imaging under defined atmospheric conditions.
What gas range does the Silver Line support?
The Silver Line system controls CO2 from 0–10% and O2 from 0.1–95% (with N2 displacement), allowing physiological normoxia (5% O2), severe hypoxia (<1% O2), hyperoxia, and standard 5% CO2 incubator conditions to be programmed and held stably.
Is the Silver Line compatible with all ibidi Stage Top Incubators?
Yes — the Silver Line Gas Incubation System interfaces with the full range of ibidi Stage Top Incubators (Universal Fit, Multi-Well Plate, K-Frame), as well as several third-party microscope stage-top chambers via custom adapters.
Can gas concentrations be changed programmatically during an experiment?
Yes — the Silver Line controller supports scripted timed transitions between gas states, enabling protocols like ischemia-reperfusion (drop O2, then restore), hypoxia-induced gene expression studies, and cyclic hypoxia/normoxia exposure during continuous time-lapse imaging.
Is the Silver Line system available with local support in Israel?
Yes — NBT Ltd is the exclusive ibidi distributor in Israel and provides installation, application guidance, and technical support for the Silver Line Gas Incubation System and its integration with researchers' existing microscopes.
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