ibidi Gas Incubation System – Silver Line
A gas mixer with active humidity control for long-term live cell imaging, providing precise CO2 and O2 regulation with patented feedback-controlled humidity control to prevent evaporation. Pairs with any ibidi Heating System to form a complete Stage Top Incubator. Catalog Number: IBD-11923

What is the ibidi Gas Incubation System – Silver Line?
The ibidi Gas Incubation System – Silver Line is a precision gas mixer and active humidity control unit designed for long-term live cell imaging on the microscope. It provides independent control of CO2, O2, and relative humidity — maintaining stable physiological conditions throughout imaging sessions when paired with an ibidi Heating System.
The system includes the ibidi Gas Mixer – Silver Line, a Humidifying Column, and an optional Air Pressure Generator. Together with the ibidi Heating System, it forms the complete ibidi Stage Top Incubator for full environmental control on any inverted microscope stage.
Key Features
- Patented Humidity Control: Feedback-controlled humidity regulation maintains a constant, very high relative humidity level inside the Stage Top Incubator, completely preventing evaporation throughout long-term live cell imaging assays.
- CO2 and O2 Regulation: Independent control of CO2 (0–15%) and O2 (1–21%) with 0.1% resolution enables normoxia, hypoxia, and custom atmospheric conditions for demanding cell biology experiments.
- Compatible with All ibidi Heating Systems: Fully compatible with the ibidi Heating System Slide/Dish, ibidi Heating System Multiwell Plate, and ibidi Heating System 4 Slides — all Silver Line versions.
- Compact and Lab-Ready: The ibidi Gas Mixer (90 × 170 × 230 mm, 2230 g) fits easily on the lab bench and connects to standard pressurized air and CO2/N2 supply at 1 bar.
- Touch Display Compatible: Integrates with the optional ibidi Touch Display for real-time monitoring and data logging of CO2, O2, and humidity during incubation.
- Scalable Platform: Use as a standalone gas regulation unit or as part of the complete ibidi Stage Top Incubator for full atmospheric control on the microscope stage.
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Technical Specifications
ibidi Gas Mixer – Silver Line
| CO2 Control Range | 0–15% |
| CO2 Resolution | 0.1% |
| O2 Control Range | 1–21% |
| O2 Resolution | 0.1% |
| Flow Control (Mixed Gas) | 0–20 l/h |
| Humidity Control Range | 20–99% relative humidity |
| Humidity Resolution | 0.1% |
| Operating Temperature | 18–30°C / 64–86°F |
| Max. Operating Humidity | 80% relative humidity |
| Dimensions (w × l × h) | 90 × 170 × 230 mm |
| Weight | 2230 g / 4.9 lbs |
| Power Supply | AC 100–240 V, 50/60 Hz, 1.4 A |
| Gas Supply – Air | 1 bar optimum (0.4–1.2 bar / 5.8–17.4 psi) |
| Gas Supply – CO2 | 1 bar optimum (0.8–1.2 bar / 11.6–17.4 psi) |
Humidifying Column
| Max. Operating Humidity | 80% relative humidity |
| Dimensions (w × l × h) | 70 × 110 × 300 mm |
Applications
- Long-term live cell imaging under controlled CO2 and O2 atmospheres
- Hypoxia and normoxia experiments on the microscope stage
- Fluorescence, confocal, widefield, and TIRF microscopy
- Cell culture maintenance during live imaging assays
- Combined use with ibidi Heating Systems for complete stage top incubation
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Cell Cultivation and Imaging
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Explore All Productsibidi Gas Incubation System Publications
The ibidi Gas Incubation System is cited in studies requiring precise atmospheric control during live cell microscopy. Researchers use it for hypoxia assays, CO2/O2 regulation experiments, and long-term time-lapse imaging under physiological conditions.

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