Applications
- Live cell imaging that requires incubator conditions on the microscope, for example:
– Chemotaxis
– Angiogenesis
– Cell migration, proliferation, wound healing
– Cell culture under flow - Imaging applications with extra focus stability, for example:
– TIRF
– Confocal microscopy - Anaerobic conditions or oxidative stress
Rat fibroblasts (cell line)
Specifications
| Temperature Controller | |
| Operating humidity | Max. 80% relative humidity (RH) |
| Environmental operating temperature | 18–30°C/64–86°F (min 5°C/9°F less than set temperature) |
| Dimensions | 90 mm x 170 mm x 230 mm |
| Weight | 1720 g / 3.8 lbs |
| External power supply | AC 100–240 V, 50/60 Hz, 2 A |
| Heated Plate and Lid | |
| Plate dimensions | 127.5 x 85.5 x 12 mm³ (fits standard multiwell holders) |
| Lid dimensions | 127.5 x 85.5 x 19 mm³ |
| Height of the system | 25.5 mm |
| Weight of incubation chamber with insert | 330 g / 0.46 lbs |
| Gas inlet | Luer connector with Luer Lock |
| Restrictions for objective lenses | |
| Optical: | None |
| Geometrical: | Possible collision with large objectives near the edges, narrowing the field of view |
| Custom openings | On request |
Heating Stages for Live Cell Imaging – No Condensation
The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.
By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.
Stable and Homogeneous Temperature
Stable and consistent temperature distribution in the µ-Slide VI 0.4 (left) and the µ-Slide 8 Well (right) during incubation in the ibidi Heating System, Universal Fit. Images were acquired with a FLIR thermal camera.
Components of the ibidi Heating & Incubation System, Universal Fit
ibidi Temperature Controller
The Temperature Controller controls the temperature of the different heated components (Heated Lid and Heated Plate). Four control channels are available.
Technical Features:
- Temperature stability: ± 0.05 °C
- Temperature uniformity across the insert: ± 0.5 °C
- Temperature range: Room temperature – 45 °C
- Control channels: 4
- IncuControl software for remote control and data recording included
- External temperature sensor included
- Adjustable alarm function included
- Analog output for extreme noise reduction optional
Heated Plate in a Multiwell Format for 1 Chamber
- Can be used with any inverted microscopy system
- Fits into both manual and automated stages
- Can be placed into any regular multi-well frame that comes with your microscope
- Adaptable to all types of microscopy chambers, such as µ-Slides and µ-Dishes plus non-ibidi formats
Heated Plate in a Multiwell Format for 4 µ-Slides
- Only included in the ibidi Heating System, Universal Fit, for 4 µ-Slides
- Can be used with any inverted microscopy system
- Ideal for use with motorized stages
- Can be placed into any regular multi-well frame that comes with your microscope
- Compatibe with all ibidi µ-Slides and non-ibidi slide formats
Heated Glass Lid, Universal Fit, for Use With CO2 and Humidity
- For use with CO2, O2, and humidity to provide a complete environmental chamber
- Independently controlled glass lid, warmed to 40 °C, prevents the formation of condensed water on the lid of the microscopy chamber or plate
- No scattering of light or shadow formation
Heating Inserts
- For all ibidi µ-Slides
- For µ-Dish 35mm. high
- For µ-Dish 35mm. low
- For LabTek TM chambered coverglass
- To be used with the Heated Plate in Multi-Well Format for 1 chamber
Optional: Heating Insert Adapter for Perfusion Assays
- To be used with the ibidi Pump System for live cell imaging under flow conditions
Manufacturer
