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ibidi 8 Well Chamber, removable
A removable silicone chamber with eight wells bonded to a standard microscope glass slide. Cultivate, fix, stain and image without ever transferring cells, then peel the chamber away to leave an ordinary glass slide that can be coverslipped, imaged on an upright microscope and archived in a slide box like any conventional histology preparation.
What is the ibidi 8 Well Chamber, removable?
The ibidi 8 Well Chamber is a silicone chamber with eight wells bonded to a standard microscope glass slide. The defining feature is in the name: once the experiment is finished, the silicone chamber peels away and leaves the cells attached to an ordinary glass slide, ready to be coverslipped and archived like any conventional histology preparation.
That solves a specific problem. Chambered coverslips such as the µ-Slide family are excellent for culture and imaging, but the chamber is permanent — the sample stays in a plastic vessel forever, cannot be used on an upright microscope, and does not slot into a slide box. The removable chamber gives you the convenience of multi-well culture during the experiment and a standard slide afterwards.
Cultivation, fixation, staining and imaging all happen on the same slide, so cells are never transferred between vessels. That matters most with fragile or low-abundance samples, where every transfer step costs material.
Key Features
- Chamber removes after culture: Peel off the silicone and you are left with cells on a plain glass slide, ready for coverslipping and long-term archiving.
- Upright and inverted microscopy: Once coverslipped it behaves as a conventional slide, so it works on upright stands — something chambered coverslips cannot offer.
- No cell transfer required: Culture, fix, stain and image in one place, avoiding the sample loss and disturbance that transfer steps introduce.
- Eight independent wells: Run eight conditions or replicates per slide, with the silicone walls preventing cross-contamination between wells.
- Standard glass slide format: Fits slide boxes, staining racks, archive storage and conventional slide handling equipment.
- Long-term sample storage: Finished preparations can be stored like any other stained histology slide.
- Made in Germany: Manufactured under ISO 13485.
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Technical Specifications
| Catalog Number | IBD-80841 |
| Number of Wells | 8 |
| Chamber | Removable silicone chamber |
| Bottom / Surface | Standard microscopy glass slide, sterilized |
| Pack Sizes | 15 (individually packed), 90 (individually packed), 120 (8 per tray) |
| Microscopy Configuration | Suitable for both upright and inverted microscopy |
| Suitable For | Adherent cells, immunofluorescence assays |
| Surface Note | The glass surface is hydrophobic and permits little or no cell adhesion unless additionally coated |
| Manufacturing | Made in Germany, ISO 13485 certified |
Applications
- Immunofluorescence staining across up to eight conditions per slide
- Cell culture and staining workflows requiring an archivable glass slide
- Upright microscopy of cultured adherent cells
- Histology-style sample preparation and long-term slide storage
- Co-culture and multi-condition comparisons on a single slide
- Experiments where cell transfer between vessels must be avoided
Cell Cultivation and Imaging
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Explore All Productsibidi 8 Well Chamber, removable Publications
The removable 8 Well Chamber appears in immunofluorescence and cell biology methods sections where cultured cells must end up on a conventional archivable glass slide, and in studies combining cell culture with upright microscopy or histology-style downstream handling.
When should I choose this over a µ-Slide 8 Well?
Choose the removable chamber when the sample needs to end up on a conventional glass slide — for archiving in a slide box, for imaging on an upright microscope, or for handing off to a histology workflow. Choose a µ-Slide 8 Well when you want the best possible optics for inverted high-resolution imaging and the sample does not need to leave its vessel.
Do I need to coat the glass before seeding?
In most cases yes. The glass surface is hydrophobic and supports little or no cell adhesion on its own. A coating such as collagen I, collagen IV, poly-L-lysine or fibronectin is standard practice, and ibidi Collagen Type I can be used for this. Robust immortalised lines sometimes attach adequately without coating, but primary cells generally will not.
When in the protocol should the chamber be removed?
After fixation and, typically, after staining — the wells are what keep reagents separated between conditions, so removing the chamber early defeats the purpose. Once the chamber comes off, apply mounting medium and a coverslip in the usual way.
Can I use it on an upright microscope?
Yes, and this is the main reason to choose it. With the chamber removed and a coverslip mounted, it is an ordinary glass slide, so it works on upright stands. Chambered coverslips such as the µ-Slide family are restricted to inverted microscopy because the chamber walls sit in the way.
Is the optical quality as good as a chambered coverslip?
For high-end techniques, no — and that is the trade-off. This product uses a standard microscopy glass slide, not a #1.5 or #1.5H coverslip, so it is not suited to TIRF, single molecule detection or super-resolution work. For routine immunofluorescence and standard fluorescence or brightfield imaging it is perfectly adequate. If you need high-NA performance, use the µ-Slide 8 Well Glass Bottom instead.
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