What is the R812/FR-12 Dual Color Multichannel Fiber Photometry System?

The RWD R812/FR-12 Dual Color Multichannel Fiber Photometry System is an in vivo optical recording platform optimized for red fluorescent indicators, including RCaMP, jrGECO1a, and red-spectrum neurotransmitter probes. Using 410 nm for an isosbestic reference and 560 nm for red indicator excitation, it provides high-sensitivity neuronal activity recordings in freely behaving animals across up to 9 simultaneous channels.

Red indicators offer key advantages over their green counterparts: reduced tissue autofluorescence, less spectral overlap with GFP-tagged viral vectors used in circuit tracing, and compatibility with simultaneous green-channel imaging when paired with an R821. The R812 is fully integrated with professional analysis software and includes a built-in optogenetics interface.

Key Features

• Dual Channel (410/560 nm): 410 nm isosbestic reference corrects for motion and bleaching artifacts; 560 nm excites red indicators (RCaMP, jrGECO1a) with picowatt-level detector sensitivity.
• Red Indicator Advantage: Reduced autofluorescence, less spectral interference with common fluorescent tracers (GFP, EGFP, mVenus), and compatibility with two-photon or green-channel parallel recordings.
• Up to 9 Simultaneous Channels: Record from multiple animals or multiple brain sites in a single session.
• No Programming Required: Integrated analysis software handles ΔF/F, Z-score, PETH, bleaching correction, behavior trajectory overlay, and data export without coding.
• Five Acquisition Modes: Continuous, interval, triggered, delayed, and timed acquisition with live ΔF/F monitoring.
• Built-In Optogenetic Interface: Supports synchronized stimulation and recording at the same brain site, compatible with blue-light optogenetics systems used alongside red calcium indicators.
• 4×4 Digital I/O: Four digital inputs and outputs (0–500 Hz) for electrophysiology, behavioral hardware, and TTL synchronization.