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R812/FR-12 Dual Color Multichannel Fiber Photometry System
The RWD R812/FR-12 is a dual-channel fiber photometry system optimized for red fluorescent indicators (RCaMP, jrGECO1a) with an isosbestic 410 nm reference. Its 560 nm excitation channel offers lower autofluorescence and reduced spectral overlap with GFP-based tracers, enabling cleaner recordings in complex circuit experiments across up to 9 simultaneous channels.
What is the R812/FR-12 Dual Color Multichannel Fiber Photometry System?
The RWD R812/FR-12 Dual Color Multichannel Fiber Photometry System is an in vivo optical recording platform optimized for red fluorescent indicators, including RCaMP, jrGECO1a, and red-spectrum neurotransmitter probes. Using 410 nm for an isosbestic reference and 560 nm for red indicator excitation, it provides high-sensitivity neuronal activity recordings in freely behaving animals across up to 9 simultaneous channels.
Red indicators offer key advantages over their green counterparts: reduced tissue autofluorescence, less spectral overlap with GFP-tagged viral vectors used in circuit tracing, and compatibility with simultaneous green-channel imaging when paired with an R821. The R812 is fully integrated with professional analysis software and includes a built-in optogenetics interface.
Key Features
- Dual Channel (410/560 nm): 410 nm isosbestic reference corrects for motion and bleaching artifacts; 560 nm excites red indicators (RCaMP, jrGECO1a) with picowatt-level detector sensitivity.
- Red Indicator Advantage: Reduced autofluorescence, less spectral interference with common fluorescent tracers (GFP, EGFP, mVenus), and compatibility with two-photon or green-channel parallel recordings.
- Up to 9 Simultaneous Channels: Record from multiple animals or multiple brain sites in a single session.
- No Programming Required: Integrated analysis software handles ΔF/F, Z-score, PETH, bleaching correction, behavior trajectory overlay, and data export without coding.
- Five Acquisition Modes: Continuous, interval, triggered, delayed, and timed acquisition with live ΔF/F monitoring.
- Built-In Optogenetic Interface: Supports synchronized stimulation and recording at the same brain site, compatible with blue-light optogenetics systems used alongside red calcium indicators.
- 4×4 Digital I/O: Four digital inputs and outputs (0–500 Hz) for electrophysiology, behavioral hardware, and TTL synchronization.
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Technical Specifications
| Catalog Number | R812 / FR-12 (US) |
| Excitation Wavelengths | 410 nm (reference), 560 nm (red) |
| Compatible Indicators | RCaMP, jrGECO1a, red neurotransmitter probes |
| Power Output | 0 – ≥130 µW, adjustable, 0.1 µW precision |
| Number of Channels | Up to 9 |
| Frame Rate | Max 250 fps |
| Digital Signal Interface | 4 Input + 4 Output |
| Signal Output | 0–500 Hz; adjustable pulse width and duration |
| Behavior Camera | 1920×1080 @ 30 fps / 1280×720 @ 60 fps |
| Acquisition Modes | Continuous, interval, triggered, delayed, timing |
| Optogenetics Integration | Built-in interface for same-site stimulation and recording |
Applications
- Calcium imaging with red indicators (RCaMP, jrGECO1a) in freely moving rodents
- Parallel dual-color recording with a green-channel system
- Circuit studies using GFP-based tracers alongside red calcium imaging
- Closed-loop fiber photometry + blue-light optogenetics
- Multi-animal behavioral neuroscience experiments
Fiber Photometry
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The RWD R812 Dual Color Multichannel Fiber Photometry System is used in research combining red calcium indicators (RCaMP, jrGECO1a) with circuit-tracing viral vectors, enabling simultaneous population-activity recording and anatomical labeling in freely behaving rodents.
Why choose red indicators over green?
Three reasons. Tissue autofluorescence is lower at 560 nm, there is less spectral overlap with the GFP and EGFP tracers used in circuit tracing, and the red channel leaves the green channel free if you later add a green system.
Which indicators does the R812 cover?
RCaMP, jrGECO1a and the red-spectrum neurotransmitter probes. Excitation is 410 nm for the isosbestic reference and 560 nm for the indicator, with output up to 130 microwatts at 0.1 microwatt resolution.
Can I record green and red at the same time?
Not on the R812 alone. Run it in parallel with a green system, or buy the R821, which carries 410, 470 and 560 nm in one unit.
How many channels does it run, and how fast?
Up to 9 simultaneous channels at up to 250 fps, with five acquisition modes: continuous, interval, triggered, delayed and timed.
Where do I buy the R812 in Israel?
NBT is the RWD distributor in Israel. Tell us the indicator and how many implants you need to read, and we will quote the R811, R812 or R821 accordingly.
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