Nanoliter 2020 Injector

WPI's updated, touchscreen-controlled nanoliter microinjector. Repeatable positive-displacement injections down to 0.6 nL using glass micropipettes, driven from the SMARTouch™ MICRO2T controller that also runs the UMP3 pump. One injector spans 1.1–1.5 mm OD glass via a simple front-gasket swap — the workhorse for stereotaxic viral delivery, Xenopus oocyte, zebrafish embryo, and Drosophila / insect injection.

Nanoliter 2020 Injector

What is the Nanoliter 2020 Injector?

The WPI Nanoliter 2020 is the updated, touchscreen-controlled generation of WPI's gold-standard Nanoliter 2010 injector — a positive-displacement microinjector capable of repeatable injections down to 0.6 nL using glass micropipettes. It connects directly to WPI's MICRO2T SMARTouch™ controller (the same controller that drives the UMP3 syringe pump), letting a single bench-top controller run injectors and pumps either independently or in sync.

The system relies on a precision micrometer step-motor and an oil-back-filled / sample-front-filled glass pipette, so the costly or scarce sample never touches the moving piston. Front-gasket swaps (green / black / red) let the same injector accept 1.1–1.15 mm, 1.3–1.35 mm, or 1.5 mm OD fire-polished glass — a single instrument across most rodent, zebrafish, Xenopus, Drosophila and insect injection workflows.

Key Features

  • Down to 0.6 nL repeatable injections. Positive-displacement architecture with in-depth plunger-displacement validation — nanoliter-to-microliter range with reproducible volumes and injection rates.
  • SMARTouch™ controller, shared with the UMP3 pump. One MICRO2T touchscreen controller can drive up to two devices (injector + injector, or injector + UMP3 pump) independently or synchronously.
  • Multi-glass compatibility, no head swap. Green / black / red front gaskets cover 1.1–1.5 mm OD fire-polished glass micropipettes without changing the injector head.
  • Sample-saving front-fill. Mineral oil back-fills the pipette; the sample is loaded at the tip, so even the smallest, most expensive samples are used in full — nothing is lost into the piston bore.
  • LED status indicator. Bright red in RUN mode, dim red when paired with the controller — immediate visual confirmation that the injector is connected and active.
  • Mounts directly on stereotaxic frames and micromanipulators for in-vivo viral vector / drug delivery, plus benchtop holders for zebrafish and embryo workflows.
  • Optional foot-switch trigger for hands-free injection during stereotaxic surgery.
  • Compatible with WPI MICRO2T and MICRO4 controllers — no adapter required (unlike the legacy Nanoliter 2010).

Applications

  • Stereotaxic viral vector (AAV) and drug delivery in mice and rats
  • Xenopus oocyte injection
  • Zebrafish embryo microinjection
  • Drosophila and other insect embryo / larval injection
  • Intracellular and cellular injection in cultured systems

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Specifications

Catalog (Complete System)NANOLITER2020
Catalog (Injector Head only)300704
ArchitecturePositive displacement, micrometer step-motor
Minimum repeatable volume0.6 nL
RangeNanoliter to microliter
Compatible glass OD1.1–1.15 mm (green gasket, installed) / 1.3–1.35 mm (black) / 1.5 mm (red)
ControllerWPI MICRO2T SMARTouch™ (also drives UMP3 pumps; up to 2 devices, individual or synchronous)
LED indicatorBright red = RUN mode; dim red = connected
TriggerTouchscreen and optional foot switch
MountingMicromanipulator (e.g. M3301) or stereotaxic frame (e.g. 505313)
Required accessoriesMICRO2T controller; 300746 Spare Parts Kit
Included with NANOLITER2020 systemInjector head, MICRO2T controller, tweezers (501981), glass capillary pack (504949, ID 0.530 mm / OD 1.14 mm × 300), 2× TIP10XV119 micropipettes, 300746 Spare Parts Kit

Improvements vs. Nanoliter 2010

Direct connection to MICRO2T (no adapter)Adapter required on 2010 — not needed on 2020
1.3 and 1.5 mm OD glass support2010: 1.14 mm only — 2020: 1.1–1.5 mm
LED status indicatorNot on 2010; present on 2020
Plunger-displacement precisionIn-depth validation on 2020

Applications

  • Stereotaxic viral vector / drug delivery in rats and mice
  • Xenopus oocyte injection
  • Zebrafish embryo microinjection
  • Drosophila / insect embryo / larva injection
  • Intracellular cell injection

WPI Nanoliter 2020 with MICRO2T SMARTouch controller
Nanoliter 2020 Injector Head (300704)
Nanoliter 2020 complete kit — injector, controller, accessories
Nanoliter 2020 LED RUN-mode indicator
Nanoliter 2020 hands-on stereotaxic use
Nanoliter 2020 mounted for in-vivo injection
Nanoliter 2020 gasket orientation diagram (green / black / red)
Nanoliter 2020 example application 1
Nanoliter 2020 example application 2

Animal Infusion

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Zebra Fish Research

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Peer-reviewed research using the WPI Nanoliter 2020 / Nanoliter 2010 injector

Academic papers and protocols citing the WPI Nanoliter family of nanoliter-volume microinjectors — including stereotaxic viral vector delivery in rodents, Xenopus oocyte microinjection, zebrafish embryo injection, and Drosophila / insect injection workflows.

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