What is the IOS-465/589/635 Intelligent Optogenetics System?

The RWD IOS-465/589/635 Intelligent Optogenetics System is a next-generation all-in-one optogenetics controller, combining a precision laser source and programmable waveform generator in a single integrated unit. It delivers light stimulation at six wavelengths (450, 465, 532, 589, 630, and 635 nm) to activate or silence genetically encoded light-sensitive proteins in defined neuronal populations, enabling precise, cell-type-specific control of neural circuits in freely behaving animals.

Building on the previous generation, the new system adds remote control capability (up to 10 m), one-click test conversion, and three configurable trigger modes (Edge, Real-Time, and Gate) to accommodate the full range of experimental designs - from electrophysiology co-recordings to fiber photometry and closed-loop behavioral paradigms. By minimizing experimenter proximity to the animal, the system reduces human-induced behavioral confounds and improves experimental reproducibility.

Key Features

• Remote Control (10 m range): Operate the system from a distance, eliminating researcher presence near the animal during behavioral experiments and reducing stress-related confounds.
• Three Trigger Modes: Edge mode (runs program on trigger onset), Real-Time mode (laser mirrors trigger signal duration), and Gate mode (external device controls parameters at up to 500 Hz) - compatible with electrophysiology rigs, fiber photometry systems, and closed-loop setups.
• Multiple Wavelengths: Six available wavelengths (450, 465, 532, 589, 630, 635 nm) cover all major optogenetics opsins including ChR2, C1V1, ArchT, eNpHR3.0, and Chrimson for both activation and silencing.
• High Laser Stability (<1% variation): Ensures consistent light delivery across multi-trial experiments, critical for reproducible modulation of neural activity.
• Precise Power Control: 0–100 mW output (1 mW resolution; IOS-635: 1–60 mW), with voltage input 2–10 V and output 10 mV–5 V for flexible interfacing with recording systems.
• Built-In Safety Features: Password protection, high-temperature alarm, and real-time program run indicator protect both the user and the equipment during long experimental sessions.
• Internal Memory + USB Storage: Save and recall stimulation protocols directly on the device or via USB flash drive for convenient protocol management across experiments.