Ideal for cell microscopy due to black walls and optically clear flat bottomExcellent inner well flatness and whole plate flatnessLow well-to-well crosstalk in fluorescence microscopy.

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Applications:

• Fluorescence-based imaging of living or fixed cells
• High throughput screening (HTS) in cell culture
• Compound screenings (toxicology)
• Large-scale transfection experiments

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Technical Features:

• Standard format, and dimensions of 85.5 x 127.5 mm (meets ANSI/SLAS (SBS) Standards)
• 96 quadratic wells with standard numbering
• Compatible with automation equipment
• Excellent inner well flatness and whole plate flatness
• Suitable for fluorescence scanners
• High-quality plastic bottom, which is compatible with solvents for staining and fixation, and also with immersion oil
• Sterile, single packaging

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Excellent for Cell Microscopy

Inner well flatness < 5 µm
Whole plate flatness < 25 µm

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Technical Drawing

Technical drawings and details are available in the Instructions (PDF).

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FAQ

What is the ibidi µ-Plate 96 Well Black?

The ibidi µ-Plate 96 Well Black is a black-walled, 96-well plate with square wells and a flat, optically clear bottom designed for high-throughput cell-based imaging assays. The black walls minimize fluorescence cross-talk between wells, and the flat bottom ensures uniform imaging across the entire well area.

Why is a black-walled plate preferred for fluorescence imaging?

Black walls absorb stray light and prevent cross-excitation between adjacent wells, which is especially important in high-density fluorescence assays such as cell viability screens, reporter gene assays, and multi-color immunofluorescence imaging across a 96-well format.

Is the µ-Plate 96 Well compatible with high-content screening (HCS) systems?

Yes. The ibidi µ-Plate 96 Well Black conforms to ANSI/SBS microplate standards in footprint and well spacing, making it directly compatible with automated high-content imaging platforms from Operetta, ImageXpress, IN Cell Analyzer, and similar systems.

What is the bottom material and thickness?

The µ-Plate 96 Well Black uses a polymer (cyclic olefin polymer, COP) or glass bottom option, depending on the variant. The ibidi ibiTreat surface coating promotes cell adhesion without the need for additional ECM coating in many standard cell lines. Bottom thickness conforms to ibidi’s standard optical specifications for compatibility with standard objective corrections.

Is the plate suitable for live-cell imaging and kinetic assays?

Yes. The µ-Plate 96 Well Black is compatible with stage top incubators and environmental chambers for temperature, CO2, and humidity control, making it suitable for multi-hour or multi-day kinetic assays in a high-throughput format.

Is the ibidi µ-Plate 96 Well Black available and supported in Israel?

Yes. ibidi µ-Plates are distributed and supported in Israel by NBT Ltd. Our team can assist with assay design, plate coating, imaging settings, and compatibility with your high-content imaging platform.