ibidi Stage Top Incubator Multiwell Plate – Silver Line

A stage top incubator for high-throughput live cell imaging in multiwell plates on every inverted microscope with K-frame fitting (160 × 110 mm), featuring precise temperature regulation, active humidity control, and CO2/O2 regulation. Catalog Number: IBD-12724 (CO2) / IBD-12726 (CO2/O2)

ibidi Stage Top Incubator Multiwell Plate – Silver Line

What is the ibidi Stage Top Incubator Multiwell Plate – Silver Line?

The ibidi Stage Top Incubator Multiwell Plate – Silver Line is a complete incubation system for high-throughput live cell imaging in multiwell plates on every inverted microscope with a standard K-frame fitting (160 × 110 mm). It combines the ibidi Heating System Multiwell Plate and the ibidi Gas Incubation System to deliver precise, stable control of temperature, humidity, and CO2/O2 throughout long-term imaging sessions.

Designed for motorized stage microscopy and high-content imaging workflows, the system supports ANSI/SLAS-standard multiwell plates including all ibidi µ-Plates, and eliminates evaporation through ibidi’s patented, feedback-controlled Humidity Control technology.

Key Features

  • K-Frame Compatibility: Easy installation on any inverted microscope with a standard K-frame opening (160 × 110 mm) — no special adapters required.
  • Active Humidity Control: Patented, feedback-controlled humidity regulation prevents evaporation inside the Incubation Chamber throughout long-term assays, maintaining stable media volumes and osmolarity.
  • Optimal Heat Distribution: No condensation on the Incubation Chamber lid ensures brilliant, undisturbed images across all wells during time-lapse experiments.
  • Highest Focus Stability: Heat regulation by continuous current minimizes temperature and z-position fluctuations for maximum imaging stability across the entire multiwell plate.
  • CO2 and O2 Regulation: Full atmospheric control with independent CO2 and O2 regulation (CO2/O2 version: Cat. No. IBD-12726) enables hypoxia studies and physiologically accurate cell biology experiments.
  • High-Throughput Compatible: Designed for standard multiwell plates with motorized stages, supporting high-content screening and high-resolution imaging workflows including DIC and TIRF microscopy.

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Technical Specifications

Microscope CompatibilityAll inverted microscopes with K-frame fitting (160 × 110 mm)
Multiwell Plate FormatANSI/SLAS (SBS) standard (85.5 × 127.5 mm), including ibidi µ-Plates
Temperature ControlAmbient to 40°C (via ibidi Heating System Multiwell Plate – Silver Line)
CO2 Control Range0–15% with 0.1% resolution
O2 Control Range1–21% with 0.1% resolution (Cat. No. IBD-12726 only)
Humidity Control20–99% relative humidity, feedback-controlled (patented ibidi Humidity Control)
Focus StabilityContinuous current heat regulation for minimal z-position fluctuation
Imaging ModalitiesWidefield, confocal, DIC, TIRF, high-content imaging
System Componentsibidi Gas Incubation System – Silver Line + ibidi Heating System Multiwell Plate – Silver Line
OriginMade in Germany
Catalog Number (CO2)IBD-12724
Catalog Number (CO2/O2)IBD-12726

Applications

  • High-throughput live cell imaging in multiwell plates using motorized stages
  • Long-term time-lapse microscopy under physiologic conditions (37°C, CO2, high humidity)
  • Hypoxia and oxygen depletion experiments (CO2/O2 version)
  • Fluorescence microscopy including widefield, confocal, DIC, and TIRF
  • High-content imaging and screening assays in standard multiwell formats
  • Cell culture maintenance and monitoring during live imaging sessions

ibidi Stage Top Incubator Multiwell Plate – Silver Line
ibidi Stage Top Incubator Multiwell Plate – Silver Line chamber view
ibidi Stage Top Incubator Multiwell Plate – Silver Line detail
System overview ibidi Stage Top Incubator Multiwell Plate
ibidi Stage Top Incubator Multiwell Plate on microscope stage
Condensation-free imaging with ibidi Stage Top Incubator Multiwell Plate

Cell Cultivation and Imaging

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Fluorescence Imaging

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Peer-reviewed research using the ibidi Stage Top Incubator Multiwell Plate

The ibidi Stage Top Incubator Multiwell Plate is widely cited in high-throughput live cell imaging studies. Researchers use it for long-term fluorescence microscopy, hypoxia experiments, and physiological assays in standard multiwell formats on inverted microscopes.

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