ibidi Stage Top Incubator Multiwell Plate – Silver Line

A stage top incubator for high-throughput live cell imaging in multiwell plates on every inverted microscope with K-frame fitting (160 × 110 mm), featuring precise temperature regulation, active humidity control, and CO2/O2 regulation. Catalog Number: IBD-12724 (CO2) / IBD-12726 (CO2/O2)

ibidi Stage Top Incubator Multiwell Plate – Silver Line

What is the ibidi Stage Top Incubator Multiwell Plate – Silver Line?

The ibidi Stage Top Incubator Multiwell Plate – Silver Line is a complete incubation system for high-throughput live cell imaging in multiwell plates on every inverted microscope with a standard K-frame fitting (160 × 110 mm). It combines the ibidi Heating System Multiwell Plate and the ibidi Gas Incubation System to deliver precise, stable control of temperature, humidity, and CO2/O2 throughout long-term imaging sessions.

Designed for motorized stage microscopy and high-content imaging workflows, the system supports ANSI/SLAS-standard multiwell plates including all ibidi µ-Plates, and eliminates evaporation through ibidi’s patented, feedback-controlled Humidity Control technology.

Key Features

  • K-Frame Compatibility: Easy installation on any inverted microscope with a standard K-frame opening (160 × 110 mm) — no special adapters required.
  • Active Humidity Control: Patented, feedback-controlled humidity regulation prevents evaporation inside the Incubation Chamber throughout long-term assays, maintaining stable media volumes and osmolarity.
  • Optimal Heat Distribution: No condensation on the Incubation Chamber lid ensures brilliant, undisturbed images across all wells during time-lapse experiments.
  • Highest Focus Stability: Heat regulation by continuous current minimizes temperature and z-position fluctuations for maximum imaging stability across the entire multiwell plate.
  • CO2 and O2 Regulation: Full atmospheric control with independent CO2 and O2 regulation (CO2/O2 version: Cat. No. IBD-12726) enables hypoxia studies and physiologically accurate cell biology experiments.
  • High-Throughput Compatible: Designed for standard multiwell plates with motorized stages, supporting high-content screening and high-resolution imaging workflows including DIC and TIRF microscopy.

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Technical Specifications

Microscope CompatibilityAll inverted microscopes with K-frame fitting (160 × 110 mm)
Multiwell Plate FormatANSI/SLAS (SBS) standard (85.5 × 127.5 mm), including ibidi µ-Plates
Temperature ControlAmbient to 40°C (via ibidi Heating System Multiwell Plate – Silver Line)
CO2 Control Range0–15% with 0.1% resolution
O2 Control Range1–21% with 0.1% resolution (Cat. No. IBD-12726 only)
Humidity Control20–99% relative humidity, feedback-controlled (patented ibidi Humidity Control)
Focus StabilityContinuous current heat regulation for minimal z-position fluctuation
Imaging ModalitiesWidefield, confocal, DIC, TIRF, high-content imaging
System Componentsibidi Gas Incubation System – Silver Line + ibidi Heating System Multiwell Plate – Silver Line
OriginMade in Germany
Catalog Number (CO2)IBD-12724
Catalog Number (CO2/O2)IBD-12726

Applications

  • High-throughput live cell imaging in multiwell plates using motorized stages
  • Long-term time-lapse microscopy under physiologic conditions (37°C, CO2, high humidity)
  • Hypoxia and oxygen depletion experiments (CO2/O2 version)
  • Fluorescence microscopy including widefield, confocal, DIC, and TIRF
  • High-content imaging and screening assays in standard multiwell formats
  • Cell culture maintenance and monitoring during live imaging sessions

ibidi Stage Top Incubator Multiwell Plate – Silver Line
ibidi Stage Top Incubator Multiwell Plate – Silver Line chamber view
ibidi Stage Top Incubator Multiwell Plate – Silver Line detail
System overview ibidi Stage Top Incubator Multiwell Plate
ibidi Stage Top Incubator Multiwell Plate on microscope stage
Condensation-free imaging with ibidi Stage Top Incubator Multiwell Plate

Cell Cultivation and Imaging

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Fluorescence Imaging

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Peer-reviewed research using the ibidi Stage Top Incubator Multiwell Plate

The ibidi Stage Top Incubator Multiwell Plate is widely cited in high-throughput live cell imaging studies. Researchers use it for long-term fluorescence microscopy, hypoxia experiments, and physiological assays in standard multiwell formats on inverted microscopes.

What is the Silver Line Stage Top Incubator Multiwell Plate version used for?

The Silver Line Stage Top Incubator – Multiwell Plate is designed for live-cell microscopy of cells in standard SBS-format multiwell plates (6, 12, 24, 96, 384), providing precise temperature and atmospheric control directly on the microscope stage during long-term time-lapse imaging.

Which microscope stages does the Silver Line Multiwell unit fit?

The Silver Line Multiwell incubator fits universal motorised stages from all major microscope vendors (Nikon, Olympus, Zeiss, Leica) via adaptor frames, and is compatible with inverted widefield, confocal, and spinning-disk configurations used in live-cell imaging.

What plate formats are supported?

The Silver Line Multiwell incubator accepts standard SBS footprint multiwell plates from 6-well through 384-well, including ibidi µ-Plates, Corning, Greiner, and Eppendorf plates, with appropriate lid and gasket designs for each format.

Does the Silver Line support gas control as well as temperature?

Yes — when paired with the Silver Line Gas Incubation System, the Multiwell incubator delivers full atmospheric control (CO2 0–10%, O2 0.1–95%) in addition to precise temperature regulation, supporting hypoxia studies and physiological imaging in multiwell formats.

Is the Silver Line Multiwell incubator available with local support in Israel?

Yes — NBT Ltd distributes ibidi products in Israel and provides installation, microscope integration, and ongoing technical support for the Silver Line Stage Top Incubator and its multi-well plate configurations.

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