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ibidi Mounting Medium With DAPI
A ready-to-use mounting medium with DAPI nuclear counterstain pre-mixed at working concentration, combining mounting and nuclear staining into a single step. Optimised for immunofluorescence in ibidi µ-Slides and µ-Dishes, delivering brilliant fluorescence signals with low background.
What is the ibidi Mounting Medium With DAPI?
This is the ibidi Mounting Medium with DAPI nuclear counterstain already incorporated, so mounting and nuclear staining happen in a single pipetting step. For anyone running immunofluorescence panels at volume, collapsing two steps into one removes a wash cycle, a timing variable and an opportunity for sample loss.
Like the base medium, it is formulated for the polymer and glass bottoms used in ibidi µ-Slides and µ-Dishes, delivering brilliant fluorescence signals against low background fluorescence.
Key Features
- Counterstain and mount in one step: DAPI is pre-mixed at working concentration — no separate nuclear staining incubation required.
- Brilliant signals, low background: Preserves fluorophore intensity while keeping non-specific background fluorescence minimal.
- Dropper bottle dispensing: Precise delivery into narrow channels or open wells without bubbles.
- Ready to use: No preparation, dilution or curing agent needed.
- Validated for widefield and confocal: Works across standard immunofluorescence imaging modalities.
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Technical Specifications
| Catalog Number | IBD-50011 |
| Pack Sizes | 15 ml, or 6 × 15 ml |
| Counterstain | DAPI, pre-mixed at working concentration |
| DAPI Excitation / Emission | ~358 nm / ~461 nm |
| Format | Ready-to-use, dropper bottle |
| Compatible Techniques | Widefield fluorescence, confocal microscopy, immunofluorescence |
| Manufacturing | Made in Germany, ISO 13485 certified |
Applications
- Single-step mounting and nuclear counterstaining
- Immunofluorescence assays in µ-Slides and µ-Dishes
- High-content and multi-well fluorescence screening
- Cell cycle and nuclear morphology studies
- Confocal and widefield imaging of fixed samples
Fluorescence Imaging
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Frequently cited in immunofluorescence protocols where nuclear counterstaining and mounting are combined, including high-content screening, cell cycle analysis and nuclear morphology studies in ibidi chambered coverslips.
Do I still need a separate DAPI staining step?
No. DAPI is already present at working concentration, so applying the medium stains nuclei and mounts the sample simultaneously. This removes an incubation and a wash cycle from the protocol, which is a meaningful saving when processing many wells.
Which filter set do I need to visualise the DAPI signal?
A standard DAPI filter set works: excitation around 358 nm and emission around 461 nm. Any blue channel configured for Hoechst or DAPI on a widefield or confocal system will image it without modification.
Will the DAPI interfere with other fluorophores in my panel?
DAPI occupies the blue channel, so it will conflict with any other blue-emitting dye such as Alexa Fluor 405 or CFP. If your panel already uses that channel, use the standard ibidi Mounting Medium without DAPI instead.
How soon after applying can I image?
Nuclei are usually well stained within a few minutes of application. Allowing five to ten minutes before imaging gives a more uniform signal, particularly in thicker samples where the dye needs time to penetrate.
Can it be used with the ibidi µ-Plate formats for screening?
Yes. The single-step workflow is particularly useful in 96-well and 384-well µ-Plates, where eliminating a separate staining and washing round meaningfully reduces both handling time and well-to-well variability.
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