Rat fibroblasts (cell line)

Find Out More

Please find more detailed information about the planning, conduction, and data analysis of live cell imaging assays here.

Download the whole “Live Cell Imaging” Application Guide as a PDF here.


Temperature Controller
Operating humidity Max. 80% relative humidity (RH)
Environmental operating temperature 18–30°C/64–86°F (min 5°C/9°F less than set temperature)
Dimensions 90 mm x 170 mm x 230 mm
Weight 1720 g / 3.8 lbs
External power supply AC 100–240 V, 50/60 Hz, 2 A
Heated Plate and Lid
Plate dimensions 127.5 x 85.5 x 12 mm³
(fits standard multiwell holders)
Lid dimensions 127.5 x 85.5 x 19 mm³
Height of the system 25.5 mm
Weight of incubation chamber with insert 330 g / 0.46 lbs
Gas inlet Luer connector with Luer Lock
Restrictions for objective lenses
Optical: None
Geometrical: Possible collision with large objectives near the edges, narrowing the field of view
Custom openings On request

Heating Stages for Live Cell Imaging – No Condensation

The independently controlled, heated glass lid of the ibidi Heating & Incubation System solves the problem of condensation in live cell imaging.

By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.

Stable and Homogeneous Temperature

Stable and consistent temperature distribution in the µ-Slide VI 0.4 (left) and the µ-Slide 8 Well (right) during incubation in the ibidi Heating System, Universal Fit. Images were acquired with a FLIR thermal camera.

Components of the ibidi Heating & Incubation System, Universal Fit

ibidi Temperature Controller

The Temperature Controller controls the temperature of the different heated components (Heated Lid and Heated Plate). Four control channels are available.

Technical Features:

  • Temperature stability: ± 0.05 °C
  • Temperature uniformity across the insert: ± 0.5 °C
  • Temperature range: Room temperature – 45 °C
  • Control channels: 4
  • IncuControl software for remote control and data recording included
  • External temperature sensor included
  • Adjustable alarm function included
  • Analog output for extreme noise reduction optional

Heated Plate in a Multiwell Format for 1 Chamber

  • Can be used with any inverted microscopy system
  • Fits into both manual and automated stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Adaptable to all types of microscopy chambers, such as µ-Slides and µ-Dishes plus non-ibidi formats

Heated Plate in a Multiwell Format for 4 µ-Slides

  • Only included in the ibidi Heating System, Universal Fit, for 4 µ-Slides
  • Can be used with any inverted microscopy system
  • Ideal for use with motorized stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Compatibe with all ibidi µ-Slides and non-ibidi slide formats

Heated Glass Lid, Universal Fit, for Use With CO2 and Humidity

  • For use with CO2, O2, and humidity to provide a complete environmental chamber
  • Independently controlled glass lid, warmed to 40 °C, prevents the formation of condensed water on the lid of the microscopy chamber or plate
  • No scattering of light or shadow formation

Heating Inserts

Optional: Heating Insert Adapter for Perfusion Assays

  • To be used with the ibidi Pump System for live cell imaging under flow conditions


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