ibidi µ-Slide 18 Well Glass Bottom

Applications

Cultivation and high-resolution microscopy of cells
TIRF and single molecule applications of living and fixed cells
Super-resolution microscopy
Immunofluorescence staining and fluorescence microscopy of living and fixed cells
Live cell imaging over extended time periods
Transfection assays
Differential interference contrast (DIC) microscopy when used with a DIC lid

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Outer dimensions (w x l)	25.5 x 75.5 mm²
Number of wells	18
Dimensions of wells (w x l x h)	5.7 x 6.1 x 6.8 mm³
Volume per well	100 µl
Height with/without lid	8.2/6.8 mm
Growth area per well	0.34 cm²
Coating area per well	1.15 cm²
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm

Technical Drawing

Technical drawings and details are available in the Instructions (PDF).

Technical Features

Chambered glass coverslip with 18 independent wells
Bottom made from D 263 M Schott glass, No. 1.5H (170 +/- 5 µm)
May require coating to promote cell attachment
Imaging chamber slide with excellent optical quality for high-end microscopy
Closely fitting lid for low evaporation
Individual well walls for minimizing well-to-well crosstalk and contaminations
Compatible with staining and fixation solutions
Also available with an ibidi Polymer Coverslip Bottom for superior cell growth: µ-Slide 18 Well
Also available as an adhesive version without a bottom: sticky-Slide 18 Well

Application Examples

Multiple conditions on one µ-Slide: For immunofluorescence stainings, toxicological screenings, cell surface coatings.

Fluorescence microscopy of rat fibroblasts (Rat1) in a µ-Slide 18 Well Glass Bottom. Red: alpha-tubulin, green: F-actin, stained with LifeAct-TagGFP2 Protein; blue: nuclei (ibidi Mounting Medium with DAPI). 60x objective lens, oil immersion.