Harvard Apparatus Pump 33 DDS™ Dual Drive System Syringe Pump
The Harvard Apparatus Pump 33 DDS™ is a dual-drive syringe pump with two fully independent stepper motors enabling simultaneous bidirectional operation from 1.02 pl/min to 106 ml/min per channel. Purpose-built for push-pull microdialysis and dual-reagent experiments, it eliminates the need for two separate pumps while offering full independent programmability per channel.

What is the Harvard Apparatus Pump 33 DDS™?
The Harvard Apparatus Pump 33 DDS™ (Dual Drive System) is a uniquely engineered dual-channel syringe pump that provides two completely independent, synchronized drive mechanisms within a single compact housing. Unlike conventional dual-syringe pumps that share a single drive motor, the Pump 33 DDS allows each channel to operate independently with its own flow rate, volume target, and direction — enabling advanced infusion/withdrawal paradigms impossible with single-drive systems.
With flow rates spanning from 1.02 pl/min to 106 ml/min per channel and a 7" LCD touchscreen interface, the Pump 33 DDS is designed for demanding applications including push-pull microdialysis, dual-reagent mixing, gradient generation, and split-flow microfluidic experiments in both animal and cell-based research.
Key Features
- Truly Independent Dual Drive System: Each of the two channels has its own independent stepper motor and drive mechanism, allowing simultaneous infusion at different flow rates, independent withdrawal, or perfectly synchronized bidirectional operation.
- Ultra-Wide Flow Range: Delivers flow rates from 1.02 pl/min to 106 ml/min per channel, supporting ultra-precise microfluidic delivery through high-volume physiological perfusion in a single instrument.
- 7" Color LCD Touchscreen: Large, intuitive touchscreen with channel-by-channel control, enabling independent programming of each drive motor from one unified screen.
- Push-Pull Microdialysis Optimized: The independent dual drives are specifically suited for push-pull perfusion — infusing artificial CSF while simultaneously withdrawing dialysate at matched flow rates, eliminating the need for two separate pumps.
- Advanced Per-Channel Programming: Supports complex multi-step methods with ramps, I/O triggers, and gradient programming per channel, all storable on-device.
- Daisy-Chain Compatible: RS-485 interface supports multi-pump daisy chaining for integration with larger automated experimental systems.
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Technical Specifications
| Catalog Number | 70-3333 |
| Drive Configuration | Dual independent drive motors |
| Flow Rate Minimum | 1.02 pl/min per channel |
| Flow Rate Maximum | 106 ml/min per channel |
| Operation Mode | Infuse and Withdraw (independent per channel) |
| Display | 7" LCD Color Touchscreen |
| Connectivity | USB, RS-485 (daisy chain), Digital I/O |
| Power Supply | 100–240 VAC, 50/60 Hz |
| Certifications | CE, ETL (UL, CSA), WEEE, EU RoHS |
Applications
- Push-pull microdialysis for in vivo neurochemical sampling
- Dual-reagent microfluidic mixing and gradient generation
- Independent dual-channel drug co-infusion studies
- Organ perfusion with simultaneous input/output flow measurement
- Cell culture perfusion with independent media and waste channels
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Harvard Apparatus Pump 33 DDS Publications
The Pump 33 DDS Dual Drive System is used in push-pull microdialysis and dual-reagent mixing studies, valued for its independent channel control enabling simultaneous infusion and withdrawal at precisely matched flow rates.

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