What is the EVOM™ Auto?

The EVOM™ Auto is WPI's automated TEER measurement system for high-throughput screening of epithelial and endothelial barrier function in 24- and 96-well HTS plates. From the global leader in TEER technology, it brings the proven EVOM™ measurement principle into a fully automated platform that minimizes human error, reduces contamination risk, and delivers a complete 96-well plate read in under four minutes.

EVOM™ Auto qualitatively measures cell monolayer health and quantitatively measures cell confluence by detecting an increase or plateau in tissue resistance, using a low AC current that is non-destructive to cells and avoids electrode polarization. The system is purpose-built for drug discovery, ADME screening, blood-brain barrier studies, and other workflows that demand reproducible TEER data at HTS scale.

Key Features

• Automated 24- and 96-well TEER: Reads a column of wells at a time using a multi-electrode array, scanning a full 96-well HTS plate in under four minutes.
• Plate Format Flexibility: One system supports Corning, Millipore, and MatTek 96-well HTS plates, plus Corning 24 and Millipore 24 HTS plates — just swap the electrode array and plate adapter.
• Three Rinse Stations: Multi-step electrode rinsing during a measurement sequence reduces carryover and lets you define your own protocol.
• Crash Protection: The system automatically detects misalignment and pauses to prevent equipment damage — protecting both the electrode array and the plates.
• Auto-Detection of Electrode Head: Plug in an electrode array and the system recognizes the format and configures itself — no manual setup.
• Browser-Based Software: Web-based control via touchscreen iPad (included), laptop, or desktop — no OS or security upgrades required.
• Multi-User Plate Profiles: Save up to three plate coordinate profiles per plate type, each date- and time-stamped, so multiple users share one instrument without overwriting each other's settings.
• Direct Data Export: All measurements stored on the instrument and exported as CSV for Excel — ready for downstream analysis.
• GxP-Ready: Optional EVOM™ GxP Compliance Module powered by TotalLab provides 21 CFR Part 11 audit trails for FDA-regulated workflows.

Applications

• Epithelial and endothelial barrier studies
• Confluence and cytotoxicity assays
• Blood-brain barrier (BBB) integrity testing
• Lung epithelial models and viral infection studies (including in vitro COVID models)
• Drug permeability and ADME screening on intestinal, kidney, and liver tissue models
• High-throughput pharmaceutical screening with optional GxP/21 CFR Part 11 compliance

System Components

Each EVOM™ Auto system includes the autosampler, an HTS electrode array (96-well, Corning 24, or Millipore 24, depending on the configuration ordered), the interface unit and cable, and a control iPad with software pre-installed.

System Configurations

Available Electrode Arrays

The same autosampler can be reconfigured for additional plate formats by purchasing extra electrode arrays — EVA-EL-03-01 (96 HTS), EVA-EL-03-02 (Corning 24 HTS), and EVA-EL-03-03 (Millipore 24 HTS). Each array ships with the matching depth-adjustment fixture and plate adapter keys.