Applications:

• Running multiple immunofluorescence assays and live cell imaging
• Real-time imaging under either static or flow conditions
• Inserting materials or tissue into perfusion channels
• Running of shear stress experiments on any substrate
• Usable with a range of bottom materials, such as plastic sheets, silicon chips, and glass slides

‍

Find Out More

Please find more detailed information about the planning, conduction, and data analysis of cell culture under flow assays here.

Download the whole “Cell Culture Under Flow” Application Guide as a PDF here.

‍

Technical Features:

• Bottomless slide
• Self-adhesive underside
• Biocompatible adhesive that has been cell culture tested
• Adheres to all flat surfaces, even wet surfaces
• Female Luer adapters for connection to tubing and pump systems
• Sterile packaging
• Suitable coverslips available
• Specifications identical to those of the µ-Slide VI ^0.4, except that it has no bottom

‍

Example: Setup of a Sample Squeezed into a Channel sticky-Slide

‍

‍

Technical Drawing

Technical drawings and details are available in the Instructions (PDF).

‍

FAQ

What is the ibidi sticky-Slide VI 0.4?

The ibidi sticky-Slide VI 0.4 is a bottomless six-channel flow slide with a self-adhesive underside, allowing researchers to mount their own substrate — such as a custom coverslip, filter membrane, or coated surface — directly beneath the channels. The 0.4 channel height (400 µm) makes it suitable for cell biology and flow experiments requiring access to custom surfaces.

What substrates can be mounted onto the sticky-Slide?

The sticky-Slide VI 0.4 can be bonded to standard coverslips (thickness #1 or #1.5), filter membranes (e.g., Transwell inserts cut to size), PDMS surfaces, ECM-coated substrates, and other flat surfaces up to approximately 300 µm thick. This makes it particularly useful when a specific coating or material must serve as the cell culture surface.

Is the sticky-Slide VI 0.4 compatible with the ibidi Pump System?

Yes. The ibidi sticky-Slide VI 0.4 is fully compatible with the ibidi Pump System and Pump System Quad via standard Luer connectors. Shear stress can be applied to cells cultured on the custom substrate mounted to the underside of the slide.

Can the sticky-Slide be used for immunofluorescence staining?

Yes. After the experiment, cells can be fixed and stained directly in the sticky-Slide. The channels can be washed and filled with antibody solutions, stains, and mounting media. The entire slide can then be imaged on an inverted microscope using the mounted coverslip.

What microscopy techniques are compatible with the sticky-Slide VI 0.4?

Widefield fluorescence, confocal, spinning disk, and — when mounted on a #1.5 coverslip — TIRF and super-resolution microscopy are all compatible. The choice of mounted substrate and its thickness determines optical suitability for high-NA objectives.

Is the ibidi sticky-Slide VI 0.4 available and supported in Israel?

Yes. The ibidi sticky-Slide VI 0.4 is distributed and supported in Israel by NBT Ltd. Our team can advise on substrate selection, bonding protocols, flow setup, and staining workflows for your specific application.