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ibidi Culture-Insert 3 Well
Three silicone wells in a row inside a 35 mm imaging dish. Seed all three, let the cells attach, remove the insert and two cell free gaps of 500 µm are left side by side. Seed the outer wells differently and one middle population meets two migration fronts in the same dish, in the same medium, in the same imaging run.
What is the Culture-Insert 3 Well?
Three silicone wells in a row, 8.4 x 12.15 x 5 mm, pre-inserted into a µ-Dish 35 mm, high with an ibiTreat surface and a #1.5 polymer coverslip bottom. Seed 70 µl per well at 3 to 7 x 105 cells/ml, wait for attachment, remove the insert. Two cell free gaps of 500 µm ± 100 µm are left, side by side in one dish.
Two gaps in one dish is the point. Seed the two outer wells differently and the middle population meets two migration fronts, both imaged in the same run under the same conditions.
Why the insert rather than a pipette tip
A scratch removes coating and leaves debris along both edges, and the gap width depends on the hand holding the tip. The moulded wells give 500 µm every time and the substrate under the gap has never been touched.
Where I would not spend the money
If one gap is enough, the 2 Well costs less and does the same thing. If you want four gaps arranged as a cross, that is the 4 Well. For many conditions rather than many gaps, the Culture-Insert 2 Well 24 in a µ-Plate 24 Well is the better buy.
Key Features
- Two cell free gaps of 500 µm ± 100 µm in one 35 mm dish
- Growth area 0.22 cm² per well, 70 µl per well
- Three separately seedable wells for co-culture and paired conditions
- ibiTreat surface on a #1.5 polymer coverslip bottom, 180 µm
- Sterilized, single use, 30 or 200 per box
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| Catalog number | 80366 |
| Number of wells | 3 |
| Number of gaps | 2 |
| Width of cell free gap | 500 µm ± 100 µm |
| Growth area per well | 0.22 cm² |
| Coating area per well | 0.82 cm² |
| Volume per well | 70 µl |
| Insert outer dimensions | 8.4 x 12.15 x 5 mm |
| Insert material | Biocompatible silicone |
| Dish | µ-Dish 35 mm, high |
| Coverslip | #1.5 polymer, 180 µm (+10/-5 µm) |
| Surface | ibiTreat, tissue culture treated |
| Recommended cell concentration | 3 to 7 x 105 cells/ml |
| Seeding volume per well | 70 µl |
| Sterilization | Sterilized, welded in gas permeable packaging, single use |
| Storage | RT (15 to 25 °C), dry, no direct sunlight |
| Shelf life | 36 months |
| Pieces per box | 30 or 200, individually packed |
Applications
- Wound healing assays
- Cell migration and invasion assays
- Co-culture migration assays
- Live cell imaging
- Immunofluorescence staining
Cell Cultivation and Imaging
Explore All ProductsFluorescence Imaging
Explore All Productsibidi Labware Publications
Comprehensive collection of peer-reviewed research publications demonstrating the use of ibidi labware including µ-Slides, µ-Dishes, µ-Plates, and specialized microfluidic devices for cell biology, neurobiology, vascular biology, and cancer research.
How many gaps do I get?
Two, both 500 µm ± 100 µm, in one µ-Dish 35 mm, high.
What does the third well buy me over the 2 Well?
The middle well is shared. Seed the two outer wells with different cell types or different treatments and both migration fronts advance into the same central population, in one dish, in one medium. With two separate 2 Well dishes you are comparing across dishes.
Seeding volume and density?
70 µl per well at 3 to 7 x 10^5 cells/ml. Growth area is 0.22 cm² per well and coating area 0.82 cm², because the coating runs under the silicone walls.
Can I use it on my own substrate?
Not this item. This is the insert already sitting in a µ-Dish. For your own coverslip or dish, order 80369, the pack of 25 Culture-Inserts 3 Well for self insertion.
Is it available in Israel with local support?
Yes. NBT is the ibidi distributor in Israel. We hold labware locally, and we will tell you when a cheaper item in the range does the same job.
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