µ-Dish 35 mm, high Glass Bottom
A 35 mm imaging dish with a glass bottom for use in TIRF, single molecule and super-resolution microscopy applications

Technical Features
- Standard format imaging dish with a 35 mm diameter for tissue culture
- Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
- May require coating to promote cell attachment
- High walls with a standard height for easy handling
- Lid with locking feature for minimal evaporation
- Rim for easy opening
- No autofluorescence
- Fully biocompatible materials
- Available as a Bulk Box with 400 individually packed µ-Dishes per box
- Available with a #1.5 ibidi Polymer Coverslip Bottom for optimized adhesion
- Available with a 50 and 500 µm grid for cell counting
Test our everyday solution for cost-effective high-throughput experiments: Glass Bottom Dish 35 mm


The Coverslip Bottom
The µ-Dish 35 mm, high Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom made from D 263 M Schott borosilicate glass that has the highest optical quality. ibidi developed these glass surfaces specifically for TIRF, super-resolution microscopy, and single molecule microscopy.

Lid with Locking Feature for Minimized Evaporation
All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, providing excellent conditions for long-term studies in a non-humidified environment. Gas exchange during cell culture is maintained thanks to the gas-permeable plastic material.






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Technical Specifications
| Dish Diameter | 35 mm |
| Volume | 2 ml |
| Growth Area | 3.5 cm² |
| Coating Area (400 µl) | 4.1 cm² |
| Observation Area Diameter | 21 mm |
| Height (with lid / without lid) | 14 mm / 12 mm |
| Bottom Material | Glass Coverslip No. 1.5H, D 263 M Schott borosilicate glass, 170 µm ± 5 µm |
Applications
- Cultivation and high-resolution microscopy of cells
- Total Internal Reflection Fluorescence (TIRF) and single molecule applications
- Super-Resolution Microscopy (STED, SIM, (F)PALM, (d)STORM) and Fluorescence Correlation Spectroscopy (FCS)
- Immunofluorescence staining of living and fixed cells
- Widefield and confocal fluorescence microscopy
- Live cell imaging over extended time periods
- Transfection assays
- Differential Interference Contrast (DIC) microscopy
- Cell location and counting (gridded version available)
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Cell Cultivation and Imaging
Explore All ProductsFluorescence Imaging
Explore All Productsibidi publications
Academic publications and research studies utilizing ibidi microfluidic cell culture systems, sourced from Google Scholar.
What is the µ-Dish 35 mm, high Glass Bottom and what is it used for?
The µ-Dish 35 mm, high Glass Bottom is A 35 mm imaging dish with a glass bottom for use in TIRF, single molecule and super-resolution microscopy applications.
What research applications is the µ-Dish 35 mm, high Glass Bottom suited for?
The µ-Dish 35 mm, high Glass Bottom is suited for neuroscience, behavioral science, physiology, and related life science research workflows requiring reliable, high-quality data acquisition and analysis.
What are the key technical features of the µ-Dish 35 mm, high Glass Bottom?
The µ-Dish 35 mm, high Glass Bottom (catalog number: IBD-81158) is engineered for precision performance in laboratory settings, offering user-friendly operation, robust construction, and compatibility with standard research workflows.
Is the µ-Dish 35 mm, high Glass Bottom available in Israel with local support?
Yes. NBT Ltd is the exclusive distributor of this product in Israel. Our team provides full local support including installation, application training, and technical assistance for the µ-Dish 35 mm, high Glass Bottom.
What accessories or systems is the µ-Dish 35 mm, high Glass Bottom compatible with?
The µ-Dish 35 mm, high Glass Bottom is designed to integrate with complementary instruments and software in its application domain. NBT Ltd can advise on compatible accessories and system configurations for your research needs.
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