Technical Features

• Standard format imaging dish with a 35 mm diameter for tissue culture
• Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
• May require coating to promote cell attachment
• High walls with a standard height for easy handling
• Lid with locking feature for minimal evaporation
• Rim for easy opening
• No autofluorescence
• Fully biocompatible materials
• Available as a Bulk Box with 400 individually packed µ-Dishes per box
• Available with a #1.5 ibidi Polymer Coverslip Bottom for optimized adhesion
• Available with a 50 and 500 µm grid for cell counting

Test our everyday solution for cost-effective high-throughput experiments: Glass Bottom Dish 35 mm

The Coverslip Bottom

The µ-Dish 35 mm, high Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom made from D 263 M Schott borosilicate glass that has the highest optical quality. ibidi developed these glass surfaces specifically for TIRF, super-resolution microscopy, and single molecule microscopy.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, providing excellent conditions for long-term studies in a non-humidified environment. Gas exchange during cell culture is maintained thanks to the gas-permeable plastic material.

FAQ

What is the ibidi µ-Dish 35 mm, high Glass Bottom?

The ibidi µ-Dish 35 mm, high is a 35 mm imaging dish featuring a high-quality glass bottom optimized for advanced fluorescence microscopy. The high-wall design (14 mm) allows larger fluid volumes and is particularly suited for TIRF, single molecule detection, super-resolution microscopy (STORM, PALM), and other techniques requiring a glass substrate.

Why is a glass bottom required for TIRF and super-resolution microscopy?

TIRF (Total Internal Reflection Fluorescence) and super-resolution techniques require a substrate with defined optical properties, including a specific refractive index and flatness. The glass bottom of the ibidi µ-Dish meets these optical requirements and enables the use of oil immersion objectives with numerical apertures above 1.4, which are incompatible with plastic substrates.

What volume can the ibidi µ-Dish 35 mm hold?

The high-wall version holds up to 3 mL of medium. The standard version holds up to 1.8 mL. The larger volume of the high version provides better CO2 buffering and reduces evaporation, which is beneficial for long-term live-cell imaging sessions.

Is the µ-Dish compatible with ibidi stage top incubators?

Yes. The ibidi µ-Dish 35 mm fits within ibidi’s stage top incubators and heating inserts, allowing temperature, CO2, and O2 control during high-resolution live-cell imaging experiments.

Can cells be transfected or treated directly in the µ-Dish?

Yes. The ibidi µ-Dish can be used for standard cell culture, transfection, immunofluorescence staining, and drug treatment directly before imaging. The glass bottom is compatible with all standard fixation and staining protocols.

Is the ibidi µ-Dish 35 mm, high Glass Bottom available and supported in Israel?

Yes. ibidi µ-Dishes are distributed and supported in Israel by NBT Ltd. Our team provides guidance on selecting the right ibidi consumable for your imaging technique and experimental requirements.