Applications

• Cultivation and high-resolution microscopy of cells
• Total Interference Reflection Fluorescence (TIRF) and single molecule applications
• Super-Resolution Microscopy (STED, SIM, (F)PALM, (d)STORM) and Fluorescence Correlation Spectroscopy (FCS)
• Immunofluorescence staining
• Widefield and confocal fluorescence microscopy of living and fixed cells
• Live cell imaging over extended time periods
• Transfection assays
• Differential interference contrast (DIC) when using a DIC Lid
• Cell location and counting when using the gridded version

Specifications

Technical Features

• Standard format imaging dish with a 35 mm diameter for tissue culture
• Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
• May require coating to promote cell attachment
• High walls with a standard height for easy handling
• Lid with locking feature for minimal evaporation
• Rim for easy opening
• No autofluorescence
• Fully biocompatible materials
• Available as a Bulk Box with 400 individually packed µ-Dishes per box
• Available with a #1.5 ibidi Polymer Coverslip Bottom with optimized adhesion: µ-Dish ^{35 mm, high}, for everyday use with diverse application possibilities
• Available with a 50 and 500 µm grid for cell counting: µ-Dish ^{35 mm, high} Grid-50 Glass Bottom and µ-Dish ^{35 mm, high} Grid-500 Glass Bottom

Test our everyday solution for cost-effective high-throughput experiments: Glass Bottom Dish ^{35 mm}

The Principle of the µ-Dish ^{35 mm, high} Glass Bottom

The Coverslip Bottom

The µ-Dish ^{35 mm, high} Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom made from D 263 M Schott borosilicate glass that has the highest optical quality. ibidi developed these glass surfaces specifically for TIRF, super-resolution microscopy, and single molecule microscopy. The dish is also available with an #1.5 ibidi Polymer Coverslip Bottom for optimal cell adhesion and high-resolution microscopy in a variety of assays.

As another option for cost-effective high-throughput experiments, we offer the Glass Bottom Dish ^{35 mm} with a #1.5 glass coverslip bottom.

Learn more about the coverslip bottom or the different surfaces of the ibidi chambers.

Find out more about the differences of our ibidi Dishes ^{35 mm}.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid-locking feature. The locking position minimizes evaporation, and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Surface-near F-actin network of a Dictyostelium discoideum DdLimE-GFP cell. Live cell imaging on a Glass Coverslip #1.5H using Total Interference Reflection Fluorescence (TIRF) microscopy.

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Fluorescence microscopy of fixed rat fibroblasts on a Glass Coverslip #1.5H. F-actin filaments are stained with phalloidin (green), nuclei are stained with DAPI (blue).

Fluorescent microscopy of a human breast cancer organoid derived from a tumor biopsy. The organoid was cultured and imaged in a µ-Dish ^{35 mm, high} Glass Bottom. The image was taken on an ANDOR Dragonfly High Speed confocal microscope system with a 25x objective. The image shows the expression of Ki67 (magenta), a marker of highly proliferative cells, at the bud tips. The cytoskeleton was labeled with phalloidin (F-actin, green) and the nuclei with Hoechst (blue). Image by Kai-Wen Kan, x-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung, China.

Fluorescent microscopy of a mouse small intestinal villi’s finger-like projections. These images are part of an in vivo animal model used to investigate the causes of endothelial dysfunction and microvascular obstruction. Image acquired using a µ-Dish 35 mm, high Glass Bottom on a Zeiss 880 confocal microscope with a 10x objective. Shown are red blood cells (green), cell death marker Annexin V (red), and platelets (blue). Image by Rhyll Smythe, The Heart Research Institute, University of Sydney, Sydney, Australia.

Electron microscopy of longitudinal ultra-thin sections of mouse embryonic fibroblasts (MEFs). Before fixation and processing, the cells were cultivated on an ibidi μ-Dish ^{35 mm, high} Glass Bottom. STEM imaging was done using a FEI Verios 460L. Structures are indicated by color overlays: lysosomes (red), mitochondria (cyan), ER (yellow), golgi (orange), microtubules (magenta), plasma membrane (green), nucleus (blue), actin (turquoise). Image by Christian Lamberz, University of Bonn, German Centre for Neurodegenerative Diseases (DZNE), Germany.

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