R550 Multi-Output Anesthesia Machine
Wall-mountable RWD anesthesia machine that anesthetizes 1–5 small animals simultaneously, with independent ON/OFF control per mask output and a separate induction chamber line. The high-throughput choice for rodent stereotaxic surgery cohorts and multi-animal physiology recordings. Available with isoflurane (R550IE) or sevoflurane (R550SE) vaporizers.

What is the R550 Multi-Output Anesthesia Machine?
The R550 from RWD Life Science is a multi-output, wall-mountable laboratory anesthesia machine purpose-built to deliver inhalation anesthesia to 1–5 small animals simultaneously — mice, rats, hamsters, guinea pigs, rabbits and cats up to 7 kg. Each of the five mask outputs has its own independent ON/OFF toggle, so animals can be added, removed or re-positioned mid-protocol without disturbing the others. A separate induction chamber line with its own 0–2.0 L/min flowmeter lets the technician prep the next subject while a procedure is underway.
The R550 is the workhorse anesthesia bench for high-throughput rodent surgery suites, multi-animal physiology recordings, and any pipeline where serial animals need anesthesia and recovery in the same session.
Key Features
- 5 mask outputs + 1 induction chamber: Anesthetize up to five small animals simultaneously, with the induction chamber gas flow controlled on a separate line.
- Independent per-channel ON/OFF: Each mask output is its own toggle switch — service one animal without disturbing the others.
- Independent dual flowmeters: 0–4 L/min for the vaporizer manifold and 0–2.0 L/min for the induction chamber.
- High-accuracy vaporizer: Individually tested with its own report; isoflurane (0–5%) or sevoflurane (0–8%), Easy Fill or Key Fill options.
- Long-life toggles: Gas path switches rated for 100,000+ cycles.
- 10 L/min O₂ flush: Clears residual anesthetic from tubing or induction chamber on demand.
- Flexible gas supply: Operate on oxygen or air; upgradable to portable configuration.
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Why multi-output anesthesia matters for throughput
Most modern rodent surgery pipelines (stereotaxic injections, neural implantation, behavioral surgery cohorts) require anesthetizing multiple animals per session. A single-channel anesthesia bench forces serial induction — the throughput-limiting step on the day. A multi-output system like the R550 lets a research group induce and maintain anesthesia on as many as five animals simultaneously, with a dedicated induction chamber for the next subject in queue.
The R550’s independent per-channel control matters operationally: an animal that reaches surgical depth can be moved to its surgical platform, its channel turned off, and the next animal swapped in — without interrupting anesthesia on the other four masks. The result is shorter procedure days, more reproducible inter-animal anesthesia duration, and fewer awake-animal welfare events.
Typical Use Cases
- Rodent stereotaxic surgery cohorts (AAV injection, electrode implantation, fiber implantation).
- Multi-animal physiology recording suites under inhalation anesthesia.
- Drug administration and tissue collection studies requiring batched anesthesia.
- Veterinary teaching labs where multiple stations operate simultaneously.
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Models
| R550IE | Enhanced Multi-Output Anesthesia Device — Isoflurane, Easy Fill, 0–4 L. Includes connection tubing for mask & induction chamber. |
| R550SE | Enhanced Multi-Output Anesthesia Device — Sevoflurane, Easy Fill, 0–4 L. |
Technical Specifications
| Outputs | 1 induction chamber + up to 5 masks (simultaneous) |
| Compatible Animals | Rats, mice, hamsters, guinea pigs, rabbits, cats (≤ 7 kg) |
| Vaporizer Flowmeter | 0–4 L/min O₂ |
| Induction Chamber Flowmeter | 0–2.0 L/min (independent) |
| O₂ Flush | 10 L/min push-button |
| Isoflurane Range | 0–5% (Easy Fill or Key Fill) |
| Sevoflurane Range | 0–8% |
| Concentration Accuracy | < 10% |
| Toggle Lifespan | 100,000+ switching cycles |
| Operating Temperature | 10–35 °C |
| Gas Supply | Oxygen or air |
Applications
- High-throughput rodent stereotaxic surgery
- Multi-animal in-vivo physiology recordings
- Batched drug administration studies
- Teaching and training labs
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Anesthesia
Explore All ProductsMicro-Surgery
Explore All ProductsR550 Multi-Output Anesthesia Machine Publications
The R550 is widely cited in high-throughput rodent surgery and physiology workflows where multiple animals must be anesthetized simultaneously. Typical studies describe stereotaxic injection cohorts, multi-animal imaging, and batched pharmacology protocols.

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