What is the µ-Slide 15 Well 3D?

The µ-Slide 15 Well 3D (formerly µ-Slide Angiogenesis) is a chambered coverslip with 15 wells, designed for tube formation (angiogenesis) assays, sprouting assays, immunofluorescence, and 3D cell culture in gel matrices. Its specialized "well-in-a-well" geometry uses only 10 µL of gel per well — about 10% of what standard multiwell plates require — making it both cost-effective and reproducible.

Because the gel forms a flat, meniscus-free surface, all cells sit in a single optical plane, enabling brilliant cell visualization across the entire well. The slide is compatible with a wide range of gels including Matrigel®, collagen, and agarose, and is optimized for phase contrast, fluorescence, and confocal microscopy.

Key Features

• Well-in-a-well geometry – 10 µL inner gel well + 50 µL upper medium reservoir
• No gel meniscus – cells grow in a uniform optical plane for reproducible imaging
• Cost-effective – only 10 µL of gel required per well
• Versatile gel compatibility – works with Matrigel®, collagen, agarose, fibrin, and other hydrogels
• High optical quality – #1.5 polymer coverslip bottom for phase contrast, fluorescence, and confocal microscopy (Glass Bottom variant available)
• Ready to use – sterile, individually wrapped, no precoating needed

Typical Applications

Tube formation (angiogenesis) assays with endothelial cells, sprouting assays from spheroids or aortic ring explants, 3D cell culture in gel matrices, co-culture and invasion experiments, sandwich cultures, and immunofluorescence staining of cells embedded in or on top of gels.