EthoVision® XT – Video tracking

EthoVision XT is the most widely applied video tracking software that tracks and analyzes the behavior, movement, and activity of any animal.

EthoVision® XT – Video tracking

EthoVision XT at the core of your lab

Over the last 25 years, EthoVision XT has evolved from video tracking software into a software platform. Designed to be at the core of your lab, EthoVision XT offers integration of data and automation of behavioral experiments with any animal.

Video tracking technology is versatile and we want you to have software that fits the bill for all of your experiments, from straight-forward protocols such as open field or Morris water maze testing to high-throughput protocols or the automation of optogenetics or operant conditioning tests.

Our Noldus EthoVision is awesome. We use it to track zebrafish larvae in real-time. DR. D. TOMASELLO |WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH, USA VIA SELECTSCIENCE

What is video tracking?

Video tracking means detecting an animal in a video file or from a live video feed, distinguishing it from its background, and tracking its whereabouts and movements. This is at the core of EthoVision XT.

In addition to tracking, EthoVision XT can also detect activity changes at pixel-level, ideal for studying behaviors such as freezing. You can also manually or automatically score behavioral events, such as rearing or grooming.

What you get out of it

As you can imagine the resulting coordinates and time stamps allow for many different kinds of analyses. EthoVision XT extracts a number of parameters (velocity, distance moved, time spent in a certain zone, and many, many more), and there are several ways to visualize your results, such as in the form of plots, graphs, tracks, and heatmaps.

Integration platform

When you define areas of interest in your arena, EthoVision XT relates movement, activity, and behavior to these zones, and use this information to base control of external equipment on. For example, drop a pellet as the mouse enters the correct arm of a radial arm maze.

EthoVision XT can also be used to integrate optogenetics, operant conditioning, and physiological data streams into your experiment.

Examples of recent publications

  • Iturra-Mena, A. M.; Aguilar-Rivera, M.; Arriagada-Solimano, M.; Pérez-Valenzuela, C.; Fuentealba, P. & Dagnino-Subiabre, A. (2019). Impact of stress on gamma oscillations in the rat nucleus accumbens during spontaneous social interaction. Frontiers in behavioral neuroscience, 13, 151.
  • Phillips, K. F., Santos, E., Blair, R. E. & Deshpande, L. S. (2019). Targeting Intracellular Calcium Stores Alleviates Neurological Morbidities in a DFP-Based Rat Model of Gulf War Illness. Toxicological sciences, 169(2), 567-578.
  • Sharon, G., Cruz, N. J., Kang, D. W., Gandal, M. J., Wang, B., Kim, Y. M., … & Bramer, L. M. (2019). Human Gut Microbiota from Autism Spectrum Disorder Promote Behavioral Symptoms in Mice. Cell, 177(6), 1600-1618.

Technical Specifications

EditionsEssential (single animal), Advanced (multiple animals in multiple arenas), Premium (3 software seats)
MeasuresDistance, velocity, acceleration, angular velocity, zone visits, latency, mobility states, user-defined variables
DetectionSubtraction-based and deep learning, including multiple animals. Deep learning runs on CUDA 12.2
Video sourceLive camera or prerecorded files, multi-camera configurations
Operating systemWindows 11 Professional 64-bit
Minimum PCIntel Core Ultra 7 265, 32 GB RAM, NVIDIA RTX 4060, 1 GB network card for GigE cameras, 1 TB free disk, mid or full tower for the camera PCIe card

Applications

  • Open field, plus maze, radial maze and other classic rodent tests
  • Zebrafish larvae tracking in well plates, with automatic zone definition in DanioVision
  • Home cage studies in PhenoTyper, up to 8 cages from one system
  • Insect and other small animal tracking

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EthoVision® XT – Noldus

Academic publications and research studies utilizing Noldus EthoVision XT video tracking software, sourced from Google Scholar.

What is EthoVision XT used for?

Video tracking software that tracks movement, activity and behavior of rodents, zebrafish and insects, live or from recorded video. It replaces manual scoring in classic tests like open field, plus maze and novel object recognition.

Which tests and species does EthoVision XT cover?

Rodents in open field, mazes and social tests, zebrafish larvae in well plates, and insects. The Advanced edition tracks multiple animals in multiple arenas at once.

What are the key technical features of EthoVision XT?

Measures distance, velocity, acceleration, angular velocity, zone visits, latency and mobility states. Detection is subtraction based or deep learning, and deep learning needs an NVIDIA GPU running CUDA 12.2. Runs on Windows 11 Professional 64-bit.

Is EthoVision XT available in Israel with local support?

Yes. NBT installs and supports EthoVision in Israel, and it is installed in more than 90% of the behavioral labs in the country. Training is part of the installation.

What does EthoVision XT work with?

PhenoTyper home cages, up to 8 from one system, the DanioVision chamber for larvae, UltraVox for vocalizations and The Observer XT for manual scoring on the same timeline.

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