ibidi µ-Slide 32 Well
Thirty-two wells on the 384-well plate pitch, with an integrated #1.5 Polymer or #1.5H Glass coverslip bottom. Each well holds 50 µl, with 0.12 cm² of growth area.

What is the µ-Slide 32 Well?
A chambered coverslip with 32 wells, spaced to the 384-well plate pitch so a multichannel pipette or a plate-format liquid handler moves onto it without adapting anything. The bottom is an integrated coverslip, either #1.5 Polymer or #1.5H Glass, so cells go from seeding straight to imaging with nothing to mount.
Each well holds 50 µl, with 0.12 cm² of growth area and 0.69 cm² of coating area at that volume. That is small next to ibidi's other chamber slides, the tradeoff for fitting 32 wells at 384-well spacing onto one slide footprint.
Small wells, standard geometry
The 384-well pitch is the point. Thirty-two wells is a small subset of that geometry, so the slide stays small footprint and low reagent volume while staying compatible with equipment built around the 384-well standard. Each well is ready for immunofluorescence straight in the well, fix, stain and image without transferring anything to a separate coverslip.
Where I would not spend the money
If the assay does not need 384-well spacing, a µ-Slide 18 Well or µ-Slide 8 Well high gives more area and volume per well on a slide footprint. Take the 32 Well when the pitch itself is what you need, not just the well count.
Key Features
- 32 wells on a 384-well plate pitch
- 50 µl per well, 0.12 cm² growth area
- #1.5 Polymer or #1.5H Glass integrated coverslip bottom
- ibiTreat tissue culture treated, or Untreated for your own coating
- Suitable for adherent cells, immunofluorescence, and single molecule, TIRF and super-resolution microscopy
- Made in Germany, 15, 90 or 120 pieces per box
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| Catalog number | 83206 (#1.5 Polymer, ibiTreat, 15 pcs/box) |
| Number of wells | 32 |
| Outer dimensions (w x l) | 25.5 x 75.5 mm |
| Dimensions of wells (w x l x h) | 3.4 x 3.4 x 6.8 mm |
| Volume per well | 50 µl |
| Height with/without lid | 8.2 / 7.0 mm |
| Growth area per well | 0.12 cm² |
| Coating area per well using 50 µl | 0.69 cm² |
| Coverslip options | #1.5 Polymer (180 µm +10/-5 µm), #1.5H Glass |
| Surface options | ibiTreat tissue culture treated, or Untreated hydrophobic |
| Pieces per box | 15 or 90 individually packed, or 120 at 8 per tray |
| Made in | Germany |
Applications
- High-throughput immunofluorescence staining
- Multi-condition screening with small cell numbers
- Live cell imaging
- Single molecule detection, TIRF and super-resolution microscopy on the Glass Bottom version
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Cell Cultivation and Imaging
צפו בכל המוצריםFluorescence Imaging
צפו בכל המוצריםWhat does the 384-well plate geometry actually buy me?
Compatibility with the hardware built around that pitch. A multichannel pipette or a plate reader set up for 384-well spacing lands on this slide the same way it would on a full plate, even though there are only 32 wells here. If nothing in your workflow is built around that pitch, the geometry itself does nothing for you.
What is the well volume and growth area?
50 µl per well, with 0.12 cm² of growth area and 0.69 cm² of coating area using the full 50 µl. That is small next to ibidi's other chamber slides, the tradeoff for fitting 32 wells at 384-well pitch onto one slide.
Polymer or Glass coverslip?
Polymer is the #1.5 ibidi Polymer bottom at 180 µm, and it covers standard widefield and confocal work. Glass is #1.5H at a tighter tolerance, and that is what TIRF, single molecule detection and super-resolution methods need. If you are not running one of those methods, the polymer version is the better value.
ibiTreat or Untreated?
ibiTreat is tissue culture treated for standard adherent cell attachment. Untreated is hydrophobic and needs your own coating. Most cell culture work should start with ibiTreat.
Is it available in Israel with local support?
Yes. NBT is the ibidi distributor in Israel.
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