Cell Neurobiology & Imaging

ibidi Culture-Insert 2 Well

Two silicone wells in a 35 mm imaging dish. Seed both, let the cells attach, lift the insert out and a cell free gap of 500 µm is left behind, with no debris along the edges and the coating under the gap untouched. A wound healing assay with the same starting geometry in every dish.

ibidi Culture-Insert 3 Well

Three silicone wells in a row inside a 35 mm imaging dish. Seed all three, let the cells attach, remove the insert and two cell free gaps of 500 µm are left side by side. Seed the outer wells differently and one middle population meets two migration fronts in the same dish, in the same medium, in the same imaging run.

ibidi Culture-Insert 4 Well

A round silicone insert with four wells, pre-inserted in a 35 mm imaging dish. Four separately seeded populations, and when the insert comes out, a cross of cell free gaps, 500 µm between neighbours and 1000 µm across the centre. Four conditions or four cell types, one dish, one imaging run.

ibidi micro-Insert 4 Well FulTrac

Four conical wells 0.4 mm across, 10 µl each, in a 35 mm imaging dish. One 20x field covers a whole well, so a single cell cannot leave the frame during a long imaging run. The FulTrac silicone bottom seals against a smooth dry surface, which is what stops 10 µl wicking away or evaporating.

ibidi µ-Dish 35 mm, high

The 35 mm dish the rest of the ibidi range is built on. A #1.5 polymer coverslip bottom, 180 µm, in place of a thick moulded base, 3.5 cm² of growth area, 2 ml of medium and a lid shaped to hold evaporation down. Phase contrast and DIC behave on it, which is not true of an ordinary plastic dish.

ibidi µ-Dish 35 mm Quad

One 35 mm dish divided into four moulded compartments, 0.85 cm² and 300 µl each, on the same #1.5 polymer coverslip bottom as the rest of the range. Four conditions in the incubator footprint of one dish, and the Ph+ compartment geometry keeps phase contrast usable right out to the wall.

ibidi µ-Slide 18 Well

Eighteen wells on a standard 25.5 x 75.5 mm slide footprint, 100 µl and 0.34 cm² each, on a #1.5 polymer coverslip bottom. Eighteen conditions or six in triplicate, stained in the wells and imaged in one pass with no coverslipping and no cells lost in transfer.

ibidi µ-Slide 8 Well high Glass Bottom

Eight wells on a standard slide footprint, 1.0 cm² and 300 µl each, on a No. 1.5H glass coverslip bottom of 170 µm ± 5 µm. The wells are 9.3 mm deep, so a multi step staining protocol runs without medium crossing between them. Buy this version when the method is TIRF, STORM or single molecule detection.

ibidi µ-Slide 15 Well 3D Glass Bottom

Fifteen wells for 3D culture and tube formation on a No. 1.5H glass bottom. Each well has an inner well 4 mm across and 0.8 mm deep that takes 10 µl of gel, with 50 µl of medium above it. The gel sets flat instead of forming a meniscus, so the cells sit in one focal plane. Sold until recently as the µ-Slide Angiogenesis Glass Bottom.

ibidi µ-Plate 24 Well Glass Bottom

A 24 well plate with black walls and a flat No. 1.5H glass coverslip bottom, 170 µm ± 5 µm. Wells are 14.0 mm across, 1.54 cm² and 1 ml each. ANSI/SLAS footprint, and the inner well and whole plate flatness is what lets an automated microscope hold focus across all 24 wells instead of refocusing in every one.

ibidi µ-Plate 96 Well Round Glass Bottom

A 96 well plate with black walls, round wells 6.2 mm across, and a flat No. 1.5H glass coverslip bottom of 170 µm ± 5 µm. 0.30 cm² and 200 µl per well, 9.0 mm pitch, ANSI/SLAS footprint. Round wells keep existing protocols, tip racks and liquid handlers working unchanged.

ibidi µ-Plate 96 Well Square Glass Bottom

A 96 well plate with black walls, square wells of 7.5 x 7.5 mm, and a flat No. 1.5H glass coverslip bottom of 170 µm ± 5 µm. 0.56 cm² and 300 µl per well, close to twice the growth area of the round well version, on the same ANSI/SLAS footprint and 9.0 mm pitch. More imageable area per well, and more coating reagent to fill it.