A powerful tool for deep tissue imaging at sub-micron resolution
High-resolution deep tissue imaging without being highly invasive is difficult to achieve. Therefore, a new imaging method that allows non- or minimally-invasive image acquisition in vivo and in real time with sub-cellular spatial resolution, and at sub-second sampling rates is needed.
MCI Pryer Endoscopic Objective
MCI now offers an exciting new imaging method that allows minimally- invasive image acquisition in vivo and in real time with sub-cellular spatial resolution – the MCI Pryer Endoscopic Objective (abbreviated as MCI Pryer). The MCI Pryer is a combined endoscopic objective and is compatible with any fluorescence microscopes, such as current 2 photon (2P) microscopes from Olympus and Nikon. This state-of-the-art objective allows for deep tissue access and visualisation in anaesthetised animals, awake animals with head fixation and even chronic recordings with our custom-built adaptor. If you are involved in 2 photon microscopy and 2 photon imaging then this endoscopic objective may well be perfect for you.
If you would like to read more about this subject then click here for our detailed introduction to the MCI Pryer Endoscopic Objective
- Probing tip: 5.67mm length and 2.1mm diameter
- Magnification: 70X; NA=0.7 OR 30x; NA=0.3
- Resolution = 0.3micron (can resolve organelles such as dendritic spines, filopodia, etc)
- Working distance > 300micron; VOF > 200um
- Working band: 450nm-1030nm
- Image quality: Plan Fluoro Achromatic NIR
- Compatibility: All upright fluorescence microscopes including bright field microscopes, confocal, 2P and super resolution microscopes STED
The advantages of using MCI Pryer in a nutshell
- Can be easily integrated into most (Olympus, Nikon, etc) current 2 photon microscopes, and giving excellent spatiotemporal resolution of <0.3μm
- Minimally invasive recordings from deep tissues such as brain regions (e.g. hippocampus) using anesthetized animals or awake animals that are head-fixed
- Provides access to any organ or tissue from the surface to deep structures
- Potential for longitudinal studies
- Real-time imaging
- Record dynamic changes in vivo
- Can be used to study drug uptake
- Easily tailored to your application and adapt to your needs
- Has the potential to be used in a broad-range of applications, for instance: oncology, molecular imaging, immunology, virology, botany, etc.
Frequently Asked Questions about the Pryer objective
1. How deep can the Pryer objective image?
The Pryer needle-tip of the Pryer objective is 7.85 or 5.87 mm in length, depending on which magnification objective you use. And, with a working distance of 500 or 200 µm, respectively, the Pryer objectives can image deep within the brain.
2. Can the Pryer objective be used with a cannula?
Yes, it can. In fact we highly recommend you use a cannula to enable long-term or chronic imaging.
3. Can you image neuronal activity in real time with the Pryer objective?
Yes you can. The pryer objective is a great addition to your current imaging setup enabling you to image deep inside the brain and even enables you to observe activity in sub-cellular compartments, like dendrites, in real time.