News, Articles, Blogs - NBT https://nbtltd.com/category/news/ Solutions that work guaranteed Wed, 08 Jan 2025 09:57:34 +0000 en-US hourly 1 https://nbtltd.com/wp-content/uploads/2022/06/cropped-logo_nbt_30-01-32x32.png News, Articles, Blogs - NBT https://nbtltd.com/category/news/ 32 32 New Installation at the Frenkel lab – Ariel Uni https://nbtltd.com/new-installation-at-the-bernstein-lab-haifa-uni-2/ Wed, 08 Jan 2025 09:57:33 +0000 https://nbtltd.com/?p=11378 We are happy to announce that we succesfuly installed a new Danio Vision tower, dedicated for Zebra fish larva studies.
The Danio Vision tower allows long term activity monitoring and tracking while manipulatin light, temperature and also startle responses via special tapping mechanism.
Along side the tower, we also inslltalled cameras and IR light sensors for application that needs bigger arenas, like full scale aquiriums.
Now the ball will shift to the lab that needs to on-board and start producing cool results.

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We are happy to announce that we successfully installed a new rTMS and Neuro-navigation setup, dedicated for transcranial stimulations.
The rTMS from Mag&More allows up to 100Hz stimulation frequencies while doing regular or pared pulses.
The system is also equipped with the Apollo View navigation systems that allows the researcher use a CT/MRI patient data as the background, allowing the researcher to come back to the same place for stimulation across multiply sessions, making the data acquisition more robust and repeatable.


Now the ball will shift to the lab that needs to on-board and start producing cool results šŸ™‚

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The lab website in case you want to learn more about Dr Frenkel’s work.

https://www.ariel.ac.il/wp/bmbl/


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New Installation at the Bernstein lab – Haifa Uni https://nbtltd.com/new-installation-at-the-bernstein-lab-haifa-uni/ Mon, 06 Jan 2025 15:22:15 +0000 https://nbtltd.com/?p=11337 We are happy to announce that we succesfuly installed a new Danio Vision tower, dedicated for Zebra fish larva studies.
The Danio Vision tower allows long term activity monitoring and tracking while manipulatin light, temperature and also startle responses via special tapping mechanism.
Along side the tower, we also inslltalled cameras and IR light sensors for application that needs bigger arenas, like full scale aquiriums.
Now the ball will shift to the lab that needs to on-board and start producing cool results.

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We are happy to announce that we successfully installed a new Danio Vision tower, dedicated for Zebra fish larva studies.
The Danio Vision tower allows long term activity monitoring and tracking while manipulating light, temperature and also startle responses via special tapping mechanism.
Along side the tower, we also installed camera and IR light projectors for applications that needs bigger arenas, like full scale aquariums.
Now the ball will shift to the lab that needs to on-board and start producing cool results šŸ™‚

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If you want to learn more about this special lab you can visit the lab Instagram

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And also the marine biology department web page


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Are you atending ISFN in Eilat this month? https://nbtltd.com/nbt-and-elveflow-are-collaborating-in-israel-in-the-microfluidics-field-2/ https://nbtltd.com/nbt-and-elveflow-are-collaborating-in-israel-in-the-microfluidics-field-2/#respond Thu, 17 Nov 2022 04:43:11 +0000 https://nbtltd.com/?p=9802 The society of Neuroscience is happening in Dan Eilat next week {Jan 12-14}

NBT will have a booth in the conference loby {booth #9}. come and meet us!!

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The society of Neuroscience is happening in Dan Eilat next week {Jan 12-14}

NBT will have a booth in the conference loby {booth #9}. come and meet us!!

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NBT and Elveflow are collaborating in Israel in the Microfluidics field https://nbtltd.com/nbt-and-elveflow-are-collaborating-in-israel-in-the-microfluidics-field/ https://nbtltd.com/nbt-and-elveflow-are-collaborating-in-israel-in-the-microfluidics-field/#respond Wed, 14 Sep 2022 14:26:16 +0000 https://nbtltd.com/?p=9699 We now have the capability to serve the Microfluidics field with the best solutions in the market, coming from Elveflow

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We now have the capability to serve the Microfluidics field with the best solutions in the market, coming from Elveflow

Elveflow products overview video

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MEA Multiwell Applications BY MCS https://nbtltd.com/mea-multiwell-applications/ Thu, 31 Oct 2019 10:48:44 +0000 https://nbtltd.com/?p=8044 Overview The Multiwell-MEA-System is designed to run simple, standardized experiments with high throughput, and analyze all replicates in one go. The Multiwell-MEAs are best suited for neuronal or cardiac cells, either primary or derived from stem cells. Substance Screening The main focus of the system is substance screening. Dose response curves for single or multiple […]

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Overview

The Multiwell-MEA-System is designed to run simple, standardized experiments with high throughput, and analyze all replicates in one go. The Multiwell-MEAs are best suited for neuronal or cardiac cells, either primary or derived from stem cells.

Substance Screening

The main focus of the system is substance screening. Dose response curves for single or multiple compounds are managed and automatically documented by our Multiwell-Screen software. Different concentrations can be applied cumulatively to a single well, or individually to a number of wells. Preliminary result reports for each experiment are generated at the end of each experiment and saved together with the data. See the Software section for more details about the analysis parameters.

Experiment setup dialog with multiple compounds

Cell Type Screening

Instead of applying new compounds to established cells, itā€™s also possible to screen new or modified cell types for their spontaneous activity or reaction to established substances. The flexible experiment layout enables experiments with multiple cells types and multiple substances on one plate.

Electrical or Optical Stimulation

Stimulation capability is included as standard in the Multiwell-MEA-System. Electrical stimulation in any well can be easily incorporated into the experimental protocol. For optical stimulation, an optional accessory is required, but the Multiwell-Screen software comes prepared to control and integrate it into the experimental workflow.

Optical stimulator for 24 or 96 wells

Combined Approaches with MEA Technology

By having a standard interface board for multiple headstage options, MCS gives researchers an opportunity to cost effectively utilize both the Multiwell-MEA-System as well as the regular single well MEA2100-System. This combination offers the advantage of both approaches; high channel counts per well and experimental flexibility on the single well arrays with the possibility to scale up established assays directly to the multiwell format.

Multiboot IFB can be used with MEA2100 headstage or Multiwell-MEA headstage

Application Notes and Culturing Protocols

Together with our partners, we have gathered helpful tips and culturing protocols for MEA electrophysiology which can be used with Multiwell-MEA plates. Please visit our download section.

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Why Choose CompresstomeĀ® ? The best tissue slicer in the world! https://nbtltd.com/why-choose-compresstome/ Sat, 06 Apr 2019 18:16:56 +0000 https://nbtltd.com/?p=3251 Patented compression technology and agarose embedding process tissue and eliminates chattermarks Slices over 5x faster than other market vibrating microtomes Preserves surface neurons to significantly improve patch clamping results for electrophysiology Slice thickness ranging from 4Ī¼m-2000Ī¼m Produces free floating slices for better immunohistochemistry (IHC) ā€“ lmmunohistochemistry results Fully automated slicing, so no more tedious cryostat […]

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  • Patented compression technology and agarose embedding process tissue and eliminates chattermarks
  • Slices over 5x faster than other market vibrating microtomes
  • Preserves surface neurons to significantly improve patch clamping results for electrophysiology
  • Slice thickness ranging from 4Ī¼m-2000Ī¼m
  • Produces free floating slices for better immunohistochemistry (IHC) ā€“ lmmunohistochemistry results
  • Fully automated slicing, so no more tedious cryostat cranking
  • Auto Zero-ZĀ®Ā technology reduces z-axis deflection to <1Ā Ī¼m
  • Light footprint for increased portability

Benefits of Compression

When using the CompresstomeĀ®, you will embed your tissue in agarose inside a specimen tube. The open end of the specimen tube has a slightly tapered edge. As tissue and agarose cross the edge of the tube, it gets slightly compressed. This compression helps stabilize the tissue during sectioning, and allows the CompresstomeĀ®Ā to section at speeds up to 5x faster than other market vibrating microtomes. Overall, the CompresstomeĀ®Ā provides a significant improvement on the health and longevity of live slices, and virtually eliminates chattermarks in fixed tissue slices.

Chattermarks

Above you can see the significant reduction in chattermarks in tissues slices produced with our CompresstomeĀ®Ā VF-300-0Z versus sections made by another leading market vibratome.

Electrophysiology

  • More than doubles the ratio of healthy-to-dead cells of acutely cut brain slices by preserving the upper surface layers of neurons.
  • Preserves subcellular microstructure by stabilizing tissue samples during sectioning.
  • Dramatically increases brain slice survival time in ACSF!
  • The CompresstomeĀ®Ā is ideal for getting healthy neurons for patch-clamp recording from mature mice (over 18+ months!). Check outĀ www.brainslicemethods.com

Auto Zero-ZĀ®

CompresstomeĀ®Ā slicer models marked with ā€œ-0Zā€ mean that the model has our patented Auto Zero-ZĀ®Ā technology. These vibrating heads are precisely aligned to eliminate vibrations in the Z-axis. Auto Zero-ZĀ®Ā technology helps reduce damage to surface cells on live tissue samples, and further reduce chattermarks on thin sections for improved imaging results.

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Parent-Child Interaction Therapy (PCIT) – From the Noldus Blog https://nbtltd.com/parent-child-interaction-therapy-pcit-from-the-noldus-blog/ Fri, 05 Apr 2019 13:45:20 +0000 https://nbtltd.com/?p=3163 JACQUELINE MARTINALI THURSDAY, 28 FEBRUARY, 2019 Parent-Child Interaction Therapy (PCIT) is an evidence-based treatment program for children with emotional and behavioral problems and their parents. The program aims to improve the quality of the parent-child relationship, and to changeĀ parent-child interactionpatterns. As a result, both the childā€™s behavioral problems and parentsā€™ stress level are reduced. PCIT […]

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THURSDAY, 28 FEBRUARY, 2019

Parent-Child Interaction Therapy (PCIT) is an evidence-based treatment program for children with emotional and behavioral problems and their parents. The program aims to improve the quality of the parent-child relationship, and to changeĀ parent-child interactionpatterns. As a result, both the childā€™s behavioral problems and parentsā€™ stress level are reduced.

PCIT improves parent-child relationships

To start, parents in this treatment program learn more about the relationship enhancement and discipline skills that they will be practicing in therapy sessions in an observation lab and at home with their child. PCIT includes a combination of play therapy and behavioral therapy.

PCIT helps improve family dynamics by working to reduce negative behavior and interactions, and to practice new behaviors and ways of communicating that are more encouraging and reassuring. When practiced consistently, these new skills and techniques can instill more confidence, reduce anger and aggression, and encourage better individual and interactive behavior in both parent and child.

Conducting research on parent-child interactions

Early in life, children are not capable of filling out a complete questionnaire or talking to an interviewer. In that case,Ā observing behavior can provide valuable informationĀ in combination with parental interviews.

Several of our customers have focused their research on parent-child interactions. They performed their studies in either aĀ laboratory settingĀ or a natural setting, such as a family home, to measure the behavior of the parents and their children. In both settings, the researchersĀ recorded the interactions on videoĀ andĀ coded behaviors in detail. By using video recordings, researchers did not lose any information and were able to replay the same scene as often as needed.

PCIT: Parent-child interaction

WHITE PAPER: HOW TO BUILD AN OBSERVATION LABĀ 

An observation lab is designed to allow you to observe your test participants unobtrusively, in an environment similar to your test participantā€™s natural surroundings. To get off to a good start, it is best to describe the research or tests in great detail.

Download this free ā€˜how toā€™ guide to learn more about building an observation lab.

In-home video recordings provide accurate measure

For example, Lisa Edelson and colleagues from the NestlĆ© Research Center in Sweden studied how parentsā€™ prompts to eat fruits and vegetables relate to childrenā€™s intake of these foods. The research team studiedĀ videos taken in an in-home setting. Ā By using portable recording devices, the researchers were able to observe the parent-child interactions in natural settings without the disruptive influence of having observers physically present in the room.

The study showed that parents who gave neutral prompts such as ā€˜eat your peasā€™ or ā€˜try your hot dogā€™ with a neutral or positive tone of voice were most successful.

Teaching parents to use new parenting skills

The key to successful interventions is the use of modifiable parenting practices. In the randomized controlled study by MontaƱo et al., researchers examined the relationship between positive behavior support (PBS) and dietary quality of the meals served to children between the ages of two and five years old, a critical period for the development of a dietary lifestyle.

Part of the study consisted ofĀ observing parent-child interactionĀ while carrying out various tasks, including free play and cleaning up, followed by meal preparation. The main purpose of the study was assessing parenting behavior.

Ways to support parent-child interaction

Researchers at the University of Wisconsin-Madison, USA, conducted a study toĀ explore the frequency and characterize the purpose of props used during mealtimesĀ with children with autism. Props are items that support child participation during mealtime, such as toys, typically child-friendly items, or common household objects.

Mealtime videos were uploaded intoĀ The Observer XT. Hereby, coders were able to review, visualize, and independently code observational data in the coding software. The study showed that props such as toys could help to calm a child and provide comfort, or support the childā€™s focus. In some families, props were also used as a reward to reinforce positive eating or mealtime behavior.

The Observer XT

TRY THE OBSERVER XT FOR YOURSELF

Request a FREE trial and see for yourself how easy behavioral research can be!

  • Work faster by automating tedious repeating tasks
  • Reduce costs by achieving more with less people
  • Get better data by using The Observer XTā€™s powerful analysis options

Encouraging more parent-child joint talk

The need to observe parent-child interactions in natural settings has been stressed by many researchers. An exhibit designed specifically for children is therefore ideal as a research setting. The team from Loyola University in Chicago chose a museum as the setting for their experimental and observational research. They examinedĀ the effects of parent-child conversationĀ and object manipulation on childrenā€™s learning, transfer of knowledge, and memory of the exhibit.

The invention resulted in more parent-child joint talk. Although children can learn a great deal on their own, conversations with parents have a big influence on the content, recall, and transfer of what they learn.

Behavioral Research Blog | Noldus

In this blog post, I have highlighted a number of studies conducted by some of our customers. These studies focused mostly on mealtime behaviors. If you are interested in reading more or about different topics, please have a look at ourĀ Behavioral Research Blog, or find some interesting publications in the list below.

Publications

Dishion, T.J.; Mun, C.J.; Drake, E.C.; Tein, J.Y.; Shaw, D.S. & Wilson, M. (2015). A transactional approach to preventing early childhood neglect: The Family Check-Up as a public health strategy.Ā Development and Psychopathology,Ā 27, 1647-1660.

Talbott, M.R.; Nelson, C.A.; Tager-Flusberg, H. (2013). Maternal Gesture Use and Language Development in Infant Siblings of Children with Autism Spectrum Disorder.Ā Journal of autism and developmental disorders, 1-11.

Thomassin, K.; Suveg, C. (2014). Reciprocal positive affect and well- regulated, adjusted children: A unique contribution of fathers.Ā Parenting: Science and Practice,Ā 14 (1), 28-46.

Weisman, O.; Zagoory-Sharon, O.; Feldman, R. (2014). Oxytocin administration, salivary testosterone, and father-infant social behavior.Ā Progress in Neuro-Psychopharmacology & Biological Psychiatry,Ā 49, 47-52.

Parent-Child interaction

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Short separation channels: the new trend in fNIRS – from the Artinis Blog https://nbtltd.com/short-separation-channels-the-new-trend-in-fnirs/ Fri, 05 Apr 2019 13:20:38 +0000 https://nbtltd.com/?p=3142 Short separation channels: the new trend in fNIRS BRITE,Ā OCTAMON,Ā OXYMON In this blog, we will explore the current use and advantage of short separation channels. We will clarify the reason why researchers apply short channels and give insight into the number of short channels that can be used. A brief example of the implementation of a […]

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Short separation channels: the new trend in fNIRS

In this blog, we will explore the current use and advantage of short separation channels. We will clarify the reason why researchers apply short channels and give insight into the number of short channels that can be used. A brief example of the implementation of a short channel for your data analysis in OxySoft will be given. Additionally, we provide a convenient overview of our devices which are compatible with short separation channels.


Short separation channels are the new trend in fNIRS. However, what is the functionality of such a short separation channel? Quite counter-intuitively, we will start by discussing the long channel. A long channel (also referred as deep channel) has a relative large interoptode distance and thereby is able to measure the concentration changes in hemoglobin relative deep in the tissue. Based on the principles of photon migration, the maximum measuring depth is approximately half the interoptode distance. For example, a distance of 30 mm between transmitter and receiver means you can measure 15 mm deep. By using a long, deep channel you can measure optical changes in the human brain. Since hemoglobin is the main absorber, fNIRS can measure the concentration changes of oxy- and deoxyhemoglobin.

The local hemoglobin change in human tissue consists of many different signal component; e.g. hemodynamic responses, blood pressure waves, Mayer waves, respirational changes, cardiac cycle (Zhang et al., 2007). Research focusses mainly on the hemodynamic response component of this signal. To eliminate the other signal components, one needs to filter the acquired signal.

A new strategy for isolating the hemodynamic response signal involves short separation channels. A short separation channel measures solely the extracerebral signals (Figure 1), which includes blood pressure waves, Mayer waves, respiration and cardiac cycles. The signal components of the short separation channel can be seen as the ā€œnoiseā€ in the signal of the long channel (Brigadoi and Cooper, 2015). By removing these components from the long channel, you can minimize this noise.

Figure 1: Measuring depth of a long channel and a short separation channel
Figure 1: Measuring depth of a long channel and a short separation channel

 

 

There are many different methods and algorithms to analyze a short separation channel. An easy method is to subtract the short channel from your long channel. In figure 2, an example of the correction of a long channel using short channel data of a fingertipping task in Oxysoft is given. Other more advanced analysis methods are using a static estimator, adaptive least mean square method (Sato et al., 2016) or a Kalman filter (Scarpa et al., 2013).

Figure 2: Reduction in physiological "noise" by subtracting short separation channel (extracerebral) data from long channel (extracerebral + intracerebral)

Figure 2: Reduction in physiological “noise” by subtracting short separation channel (extracerebral) data from long channel (extracerebral + intracerebral)

 

 

Furthermore, you should consider the number of short separation channel you would like to implement in your fNIRS system. There are several possibilities for short separation channels (SSC).

  • Local: Use one SSC for each long channel
  • Symmetrical-local: Use one SSC for each symmetrical channel
  • Symmetrical-region: Use one SSC for each symmetrical region
  • Global-ipsilateral: Use one SSC for each hemisphere
  • Global-contralateral: Use one SSC for the entire head

In Figure 3, a 16-channel optode template is presented to clarify the categories for using short separation channels. Each color represents a short separation channel needed for a long channel based on the different categories. In the article of Zhang et al., 2015, you can read more about the correlation between the locations of the short separation channels and the components of the signals.

Figure 3: 16-channel optode template set-up for local, symmetrical-local, symmetrical-region, global-ipsilateral and global-contralateral use of short separation channels. Each color represent a short separation channel for every category of long channels.

Figure 3: 16-channel optode template set-up for local, symmetrical-local, symmetrical-region, global-ipsilateral and global-contralateral use of short separation channels. Each color represent a short separation channel for every category of long channels.


Short+separation+channel+holder+3.jpg

Currently short separation channels are available in the OxyMon, OctaMon and the Brite system. Please contact us (askforinfo@artinis.com) if you already have a system and you would like to upgrade it with short separation channel(s).

References
Brigadoi, S., & Cooper, R. J. (2015). How short is short? Optimum sourceā€“detector distance for short-separation channels in functional near-infrared spectroscopy. Neurophotonics.
Sato, T., Nambu, I., Takeda, K., Aihara, T., Yamashita, O., Isogaya, Y., ā€¦ Osu, R. (2016). Reduction of global interference of scalp-hemodynamics in functional near-infrared spectroscopy using short distance probes. NeuroImage, 141, 120ā€“132.
Scarpa, F., Cutini, S., Scatturin, P., Dell, R., Sparacino, G., Boas, D. A., ā€¦ Villringer, A. (2002). Bayesian filtering of human brain hemodynamic activity elicited by visual short-term maintenance recorded through functional nearinfrared spectroscopy (fNIRS). IEEE Eng. Med. Biol. Mag (Vol. 8). CRC Press.
Zhang, Q., Brown, E. N., & Strangman, G. E. (2007). Adaptive filtering for global interference cancellation and real-time recovery of evoked brain activity: a Monte Carlo simulation study. Journal of Biomedical Optics.
Zhang, Y., Tan, F., Xu, X., Duan, L., Liu, H., Tian, F., & Zhu, C.-Z. (2015). Multiregional functional near-infrared spectroscopy reveals globally symmetrical and frequency-specific patterns of superficial interference. Biomedical Optics Express.

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